Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Jinan, China.
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Jinan, China; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.
Anal Chim Acta. 2023 Jan 25;1239:340662. doi: 10.1016/j.aca.2022.340662. Epub 2022 Nov 26.
It is challenging to achieve the highly sensitive detection of glucocorticoids at ultratrace levels because of the abundant hydrophilic groups in their molecules and the complexity of environmental water sample matrices. Here, a highly crystalline three-dimensional hydroxylated covalent organic frameworks (denoted by COF-301) with tetra(4-anilyl)methane (TAM) and 2,5-dihydroxyterephthalaldehyde (DHTA) as building units was constructed and proposed as adsorbent for solid phase extraction (SPE) of glucocorticoids. Theoretical studies were conducted to elucidate the potential adsorption mechanism of glucocorticoids on the COF-301. The COF-301 based SPE combined with liquid chromatography-tandem mass spectrometry provides a promising approach for the preconcentration and determination of glucocorticoids residue in water samples. Good linearity with a correlation coefficient exceeding 0.9988, low limits of detection ranging from 0.024 to 0.075 ng L and relative standard deviations below 6.68% were achieved. The proposed method was successfully applied to analyze glucocorticoids residue in actual water samples, demonstrating the prospects of this method for the determination of trace glucocorticoids.
由于糖皮质激素分子中含有丰富的亲水性基团和环境水样基质的复杂性，因此很难实现对其在痕量水平下的高灵敏度检测。在这里，构建了一种具有高结晶度的三维羟基共价有机框架（记为 COF-301），其结构单元为四（4-苯胺基）甲烷（TAM）和 2,5-二羟基对苯二甲醛（DHTA），并将其作为固相萃取（SPE）吸附剂用于糖皮质激素的萃取。通过理论研究阐明了糖皮质激素在 COF-301 上的潜在吸附机制。基于 COF-301 的 SPE 结合液相色谱-串联质谱法为水样中糖皮质激素残留的预浓缩和测定提供了一种很有前途的方法。该方法具有良好的线性关系，相关系数均大于 0.9988，检出限范围为 0.024-0.075ng L，相对标准偏差均低于 6.68%。该方法成功应用于实际水样中糖皮质激素残留的分析，表明该方法有望用于痕量糖皮质激素的测定。
