Holdeman M, Khorram O, Samson W K, Lipton J M
Am J Physiol. 1985 Jan;248(1 Pt 2):R125-9. doi: 10.1152/ajpregu.1985.248.1.R125.
The concentration of melanocyte-stimulating hormone (melanotropin; MSH) within the septal region of the brain increases during the fever, and septal injections of MSH are antipyretic. Corticotropin-releasing factor (CRF), when injected intracerebroventricularly, is also antipyretic. Using sensitive radioimmunoassays of microdissected tissue extracts, we established the presence of immunoreactive MSH (IRMSH) and CRF (IRCRF) within discrete central nervous system sites of the rabbit. Leukocytic pyrogen-induced fever and hyperthermia due to heat exposure did not alter concentrations of IRMSH or IRCRF in tissue extracted from preoptic-anterior hypothalamic or midbrain central gray regions. However, significantly greater levels of IRMSH were detected in septal extracts of febrile rabbits than in similar extracts from afebrile controls or heat-stressed animals. A significant decrease in IRCRF was detected in paraventricular nucleus extracts from febrile animals compared with extracts from afebrile controls or heat-stressed rabbits. Our results support the hypothesis that these central peptides have a role in temperature control during fever. Since no changes were detected in extracts from hyperthermic rabbits, it appears that changes in concentration of these neuropeptides within particular brain regions are specific to the febrile state and are not caused by elevation of body temperature or by nonspecific stress.
发热期间，脑隔区促黑素细胞激素（促黑素；MSH）的浓度会升高，向隔区注射MSH具有解热作用。促肾上腺皮质激素释放因子（CRF）经脑室内注射时也具有解热作用。我们采用灵敏的放射免疫分析法检测经显微切割的组织提取物，确定了家兔中枢神经系统离散部位存在免疫反应性MSH（IRMSH）和CRF（IRCRF）。白细胞致热原引起的发热以及热暴露导致的体温过高，均未改变从视前区 - 下丘脑前部或中脑中央灰质区域提取的组织中IRMSH或IRCRF的浓度。然而，与无热对照或热应激动物的类似提取物相比，发热家兔的隔区提取物中检测到的IRMSH水平显著更高。与无热对照或热应激家兔的提取物相比，发热动物室旁核提取物中检测到IRCRF显著减少。我们的结果支持这样的假说，即这些中枢肽在发热期间的体温调节中发挥作用。由于在体温过高的家兔提取物中未检测到变化，看来特定脑区中这些神经肽浓度的变化是发热状态所特有的，并非由体温升高或非特异性应激引起。
