Department of Clinical Pathology, Allergy and Clinical Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Department of Internal Medicine, Allergy and Clinical Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Viral Immunol. 2024 May;37(4):186-193. doi: 10.1089/vim.2024.0015.
Coronavirus disease 2019 (COVID-19) represented an international health risk. Variants of the interferon-induced transmembrane protein-3 () gene can increase the risk of developing severe viral infections. This cross-sectional study investigated the association between rs12252A>G single nucleotide polymorphism (SNP) and COVID-19 severity and mortality in 100 Egyptian patients. All participants were subjected to serum interleukin-6 (IL-6) determination by ELISA and rs12252 genotyping by real-time polymerase chain reaction. Of all participants, 85.0% had the rs12252 homozygous AA genotype, whereas 15.0% had the heterozygous AG genotype. None of our participants had the homozygous GG genotype. The rs12252A allele was found in 92.5% and the G allele in only 7.5%. There was no significant association ( > 0.05) between the rs12252 SNP and COVID-19 severity, intensive care unit (ICU) admission, or IL-6 serum levels. The heterozygous AG genotype frequency showed a significant increase among participants who died (32.0%) compared with those who had been cured (9.3%). The mutant G allele was associated with patients' death. Its frequency among cured participants was 8.5%, whereas in those who died was 24.2% ( = 0.024) with 3.429 odds ratio [95% confidence interval: 1.1-10.4]. In conclusion, this study revealed a significant association between the G allele variant of rs12252 and COVID-19 mortality. However, results were unable to establish a significant link between rs12252 polymorphism, disease severity, ICU admission, or serum IL-6 levels.
新型冠状病毒病 2019（COVID-19）是一种国际健康风险。干扰素诱导跨膜蛋白 3（）基因的变异可以增加严重病毒感染的风险。这项横断面研究调查了 100 名埃及患者中 rs12252A>G 单核苷酸多态性（SNP）与 COVID-19 严重程度和死亡率之间的关系。所有参与者均通过 ELISA 测定血清白细胞介素-6（IL-6），并通过实时聚合酶链反应进行 rs12252 基因分型。在所有参与者中，85.0%为 rs12252 纯合 AA 基因型，15.0%为杂合 AG 基因型。我们的参与者均无纯合 GG 基因型。 rs12252A 等位基因的频率为 92.5%，G 等位基因的频率为 7.5%。 rs12252 SNP 与 COVID-19 严重程度、入住重症监护病房（ICU）或 IL-6 血清水平之间无显著相关性（>0.05）。与已治愈的参与者（9.3%）相比，死亡的参与者中杂合 AG 基因型的频率显着增加（32.0%）。突变的 G 等位基因与患者的死亡相关。在已治愈的参与者中其频率为 8.5%，而在死亡的参与者中为 24.2%（=0.024），优势比为 3.429（95%置信区间：1.1-10.4）。总之，本研究显示 rs12252 的 G 等位基因变异与 COVID-19 死亡率之间存在显著关联。但是，结果无法确定 rs12252 多态性、疾病严重程度、入住 ICU 或血清 IL-6 水平之间存在显著联系。
