Department of Biochemistry and Microbiology, University of Chemistry and Technology, 160 00 Prague, Czech Republic.
Department of Chemistry, Faculty of Science, Humanities and Education, Technical University of Liberec, 461 17 Liberec, Czech Republic.
ACS Appl Mater Interfaces. 2024 May 22;16(20):25813-25824. doi: 10.1021/acsami.4c03642. Epub 2024 May 8.
To develop microbiologically safe nanofibrous materials, it is crucial to understand their interactions with microbial cells. Current research indicates that the morphology of nanofibers, particularly the diameter of the fibers, may play a significant role in biofilm formation and retention. However, it has not yet been determined how the fiber diameter of poly-ε-caprolactone (PCL), one of the most widely used biopolymers, affects these microbial interactions. In this study, two nanofibrous materials electrospun from PCL (PCL45 and PCL80) with different fiber diameter and characteristic distance δ between fibers were compared in terms of their ability to support or inhibit bacterial biofilm formation and retain bacterial cells. Strains of (ATCC 25922 and ATCC 8739) and (ATCC 25923 and ATCC 6538) were used as model bacteria. Biofilm formation rate and retention varied significantly between the and strains ( < 0.05) for the tested nanomaterials. In general, PCL showed a lower tendency to be colonized by the tested bacteria compared to the control material (polystyrene). Fiber diameter did not influence the biofilm formation rate of strains and 25922 ( > 0.05), but it did significantly impact the biofilm formation rate of 8739 and biofilm morphology formed by all of the tested bacterial strains. In PCL45, thick uniform biofilm layers were formed preferably on the surface, while in PCL80 smaller clusters formed preferably inside the structure. Further, fiber diameter significantly influenced the retention of bacterial cells of all the tested strains ( < 0.001). PCL45, with thin fibers (average fiber diameter of 376 nm), retained up to 7 log (CFU mL) of staphylococcal cells (100% retention). The overall results indicate PCL45's potential for further research and highlight the nanofibers' morphology influence on bacterial interactions and differences in bacterial strains' behavior in the presence of nanomaterials.
为了开发微生物安全的纳米纤维材料，了解它们与微生物细胞的相互作用至关重要。目前的研究表明，纳米纤维的形态，特别是纤维的直径，可能在生物膜形成和保留中起重要作用。然而，聚己内酯（PCL）作为最广泛使用的生物聚合物之一，其纤维直径如何影响这些微生物相互作用尚未确定。在这项研究中，比较了两种不同纤维直径和纤维之间特征距离δ的 PCL 电纺纳米纤维材料（PCL45 和 PCL80）在支持或抑制细菌生物膜形成和保留细菌细胞方面的能力。使用 （ATCC 25922 和 ATCC 8739）和 （ATCC 25923 和 ATCC 6538）作为模型细菌。对于测试的纳米材料， 菌株和 菌株的生物膜形成率和保留率差异显著（<0.05）。一般来说，与对照材料（聚苯乙烯）相比，PCL 被测试细菌定植的趋势较低。纤维直径不影响 菌株和 25922 的生物膜形成率（>0.05），但显著影响 8739 的生物膜形成率和所有测试细菌菌株形成的生物膜形态。在 PCL45 中，更喜欢在表面形成厚而均匀的生物膜层，而在 PCL80 中更喜欢在结构内部形成较小的簇。此外，纤维直径显著影响所有测试菌株的细菌细胞保留率（<0.001）。纤维直径较细的 PCL45（平均纤维直径为 376nm）保留了高达 7 个对数（CFU mL）的葡萄球菌细胞（保留率为 100%）。总体结果表明 PCL45 具有进一步研究的潜力，并强调了纳米纤维形态对细菌相互作用以及在纳米材料存在下细菌菌株行为差异的影响。
