Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Virology. 2024 Jul;595:110090. doi: 10.1016/j.virol.2024.110090. Epub 2024 May 1.
Nowadays finding the new antimicrobials is necessary due to the emerging of multidrug resistant strains. The present study aimed to isolate and characterize bacteriophages against S. aureus. Strains Huma and Simurgh were the two podovirus morphology phages which isolated and then characterized. Huma and Simurgh had a genome size of 16,853 and 17,245 bp, respectively and both were Rosenblumvirus with G + C content of 29%. No lysogeny-related genes, nor virulence genes were identified in their genomes. They were lytic only against two out of four S. aureus strains. They also were able to inhibit S. aureus for 8 h in-vitro. Both showed a rapid adsorption. Huma and Simurgh had the latent period of 80 and 60 m and the burst sizes of 45 and 40 PFU/ml and also, they showed very low cell toxicity of 1.23%-1.79% on HT-29 cells, respectively. Thus, they can be considered potential candidates for biocontrol applications.
由于多药耐药株的出现，现在有必要寻找新的抗菌药物。本研究旨在分离和鉴定针对金黄色葡萄球菌的噬菌体。分离并鉴定了两种痘病毒形态的噬菌体 Hum 和 Simurgh。Huma 和 Simurgh 的基因组大小分别为 16853bp 和 17245bp，均为 Rosenblumvirus，G+C 含量为 29%。它们的基因组中没有发现溶原相关基因或毒力基因。它们仅对 4 株金黄色葡萄球菌中的 2 株具有裂解活性。它们也能够在体外抑制金黄色葡萄球菌 8 小时。两者均表现出快速吸附。Huma 和 Simurgh 的潜伏期分别为 80 和 60m，爆发量分别为 45 和 40PFU/ml，对 HT-29 细胞的细胞毒性也非常低，分别为 1.23%-1.79%。因此，它们可以被认为是生物控制应用的潜在候选者。
