Rahiminejad Mina, Montaseri Maryam, Yousefi Mohammad Hashem, Nazifi Saeed, Wagemans Jeroen, Hosseinzadeh Saeid
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, 71946-84471, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Sci Rep. 2025 Apr 29;15(1):14958. doi: 10.1038/s41598-025-99116-3.
The emergence of antimicrobial-resistant bacteria has urged researchers to find alternatives or adjunct therapies, such as bacteriophages or phages. This study aimed to investigate the capabilities of Salmonella Typhimurium-specific phage Ariobarzanes in reducing cytotoxicity, invasion, and induction of necrosis and apoptosis caused by Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) ATCC 14,028 infection in the human small intestinal cell line Caco-2. For this purpose, cytotoxicity tests were performed, and the production of inflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor -alpha (TNF-α) was investigated in phage-treated versus non-treated samples (for 6-hour or 16-hour). The results indicate the success of Ariobarzanes in reducing cytotoxicity in a dose dependently, completely inhibiting Salmonella spp. invasion of Caco-2 cells, reducing Salmonella-induced apoptosis, and necrosis, and also reducing the production and secretion of cytokines IL-8, and TNF-α compared to the bacterial control treatment. Therefore, Ariobarzanes can be introduced as a potential candidate for controlling S. Typhimurium in the agriculture, food, and/or pharmaceutical industries.
抗菌耐药细菌的出现促使研究人员寻找替代疗法或辅助疗法，如噬菌体。本研究旨在调查鼠伤寒沙门氏菌特异性噬菌体阿里奥巴扎内斯在降低由肠炎沙门氏菌亚种肠炎血清型鼠伤寒沙门氏菌（鼠伤寒沙门氏菌）ATCC 14028感染人小肠细胞系Caco-2所引起的细胞毒性、侵袭以及坏死和凋亡诱导方面的能力。为此，进行了细胞毒性试验，并在噬菌体处理组与未处理组样本中（处理6小时或16小时）研究了炎性细胞因子白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的产生情况。结果表明，阿里奥巴扎内斯能够成功地剂量依赖性降低细胞毒性，完全抑制沙门氏菌属对Caco-2细胞的侵袭，减少沙门氏菌诱导的凋亡和坏死，并且与细菌对照处理相比，还能减少细胞因子IL-8和TNF-α的产生与分泌。因此，阿里奥巴扎内斯可作为农业、食品和/或制药行业中控制鼠伤寒沙门氏菌的潜在候选物。
