Mourali Donia, Kazdaghli Rahma, Gara-Ali Marwa, Ben-Miled Houda, Mora-Quilis Lucas, Domingo-Calap Pilar, Ben-Mahrez Kamel
Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis El Manar, El Manar II, Tunis 2092, Tunisia.
Institute for Integrative Systems Biology, I2SysBio, Universitat de Valencia-CSIC, 46980 Paterna, Spain.
Antibiotics (Basel). 2024 Dec 2;13(12):1154. doi: 10.3390/antibiotics13121154.
: is an opportunistic pathogen that causes a wide range of infections worldwide. The emergence and spread of multidrug-resistant clones requires the implementation of novel therapeutics, and phages are a promising approach. : In this study, two phages, KpTDp1 and KpTDp2, were isolated from wastewater samples in Tunisia. These phages had a narrow host range and specifically targeted the hypervirulent K2 and K28 capsular types of . Both phages have double-stranded linear DNA genomes of 49,311 and 49,084 bp, respectively. Comparative genomic and phylogenetic analyses placed phage KpTDp2 in the genus , while phage KpTDp1 showed some homology with members of the genus , although its placement in a new undescribed genus may be reconsidered. The replication efficiency and lytic ability of these phages, combined with their high stability at temperatures up to 70 °C and pH values ranging from 3.5 to 8.2, highlight the potential of these phages as good candidates for the control of hypervirulent multidrug-resistant . : Phage isolation, titration and multiplicity of infection were performed. The stability of KpTDp1 and KpTDp2 was tested at different pH and temperatures. Genomic characterization was done by genome sequencing, annotation and phylogenetic analysis. : The ability of KpTDp1 and KpTDp2 to lyse one of the most virulent serotypes of , as well as the stability of their lytic activities to pH and temperature variations, make these phages promising candidates for antibacterial control.
[病原体名称]是一种机会致病菌，在全球范围内可引起多种感染。多重耐药克隆的出现和传播需要实施新型治疗方法，而噬菌体是一种很有前景的途径。在本研究中，从突尼斯的废水样本中分离出两种噬菌体，即KpTDp1和KpTDp2。这些噬菌体宿主范围狭窄，专门针对[病原体名称]的高毒力K2和K28荚膜型。两种噬菌体均具有双链线性DNA基因组，大小分别为49,311 bp和49,084 bp。比较基因组学和系统发育分析将噬菌体KpTDp2归为[噬菌体属名称]属，而噬菌体KpTDp1与[噬菌体属名称]属的成员有一些同源性，不过其归为一个新的未描述属的分类可能需要重新考虑。这些噬菌体的复制效率和裂解能力，以及它们在高达70°C的温度和3.5至8.2的pH值范围内的高稳定性，突出了它们作为控制高毒力多重耐药[病原体名称]的良好候选者的潜力。进行了噬菌体分离、滴定和感染复数测定。在不同pH值和温度下测试了KpTDp1和KpTDp2的稳定性。通过基因组测序、注释和系统发育分析进行了基因组特征分析。KpTDp1和KpTDp2裂解[病原体名称]最具毒力血清型之一的能力，以及它们的裂解活性对pH值和温度变化的稳定性，使这些噬菌体成为抗菌控制的有前途的候选者。
