Wilcock B P
Can J Comp Med. 1979 Apr;43(2):200-6.
Twenty colostrum-fed piglets from three sows were separated from the sows 24 hours after birth and were randomly divided into five groups of four piglets each. Every piglet in each of four test groups was orally inoculated with about 10(10) colony forming units of Salmonella typhimurium, Salmonella choleraesuis var Kunzendorf or one of two isolates of Klebsiella pneumoniae. One group served as uninoculated controls. Piglets infected with K. pneumoniae developed severe diarrhea beginning about 12 hours after inoculation. They became dehydrated and weak but continued to drink. There were no morphological alterations in intestinal mucosa when piglets were killed and necropsied 48 or 72 hours after inoculation. Klebseilla pneumoniae was isolated from intestine and feces but not from liver or spleen. Piglets inoculated with S. choleraesuis became lethargic and disinterested in food by 24 hours after inoculation. Diarrhea developed by 48 hours after inoculation. Lesions at necropsy 60 or 72 hours postinoculation were subcutaneous edema, mesenteric lymphadenitis, diffuse intestinal superficial mucosal necrosis with villous atrophy, and focal deep ulceration in the ileum. Salmonella choleraesuis was isolated from all segments of intestine and from feces, liver and spleen. Piglets inoculated with S. typhimurium developed a relatively mild diarrheal disease with lesions similar to those with S. choleraesuis infection but less severe. The inoculated organism was recovered from all areas of intestine and from feces, liver and spleen. Serum from infected and control piglets had high (greater than 1:256) agglutinating titres against S. typhimurium but low titres (0 to 1:8) against S. choleraesuis. The agglutinins were assumed to originate from colostral antibodies.
从三头母猪所产的20头初乳喂养仔猪在出生后24小时与母猪分开，并随机分成五组，每组四头仔猪。四个试验组中的每头仔猪经口接种约10(10)个菌落形成单位的鼠伤寒沙门氏菌、猪霍乱沙门氏菌库岑多夫变种或肺炎克雷伯菌的两个分离株之一。一组作为未接种对照。感染肺炎克雷伯菌的仔猪在接种后约12小时开始出现严重腹泻。它们变得脱水和虚弱，但仍继续饮水。在接种后48或72小时处死并剖检仔猪时，肠黏膜无形态学改变。从肠道和粪便中分离出肺炎克雷伯菌，但未从肝脏或脾脏中分离出。接种猪霍乱沙门氏菌的仔猪在接种后24小时变得嗜睡且对食物不感兴趣。接种后48小时出现腹泻。接种后60或72小时剖检的病变为皮下水肿、肠系膜淋巴结炎、弥漫性肠道浅表黏膜坏死伴绒毛萎缩以及回肠局灶性深部溃疡。从肠道各段、粪便、肝脏和脾脏中分离出猪霍乱沙门氏菌。接种鼠伤寒沙门氏菌的仔猪发生相对较轻的腹泻病，病变与猪霍乱沙门氏菌感染相似但较轻。从肠道所有部位、粪便、肝脏和脾脏中回收接种的细菌。感染和对照仔猪的血清对鼠伤寒沙门氏菌有高（大于1:256）凝集效价，但对猪霍乱沙门氏菌的效价低（0至1:8）。这些凝集素被认为源自初乳抗体。
