Ellis J A, DeMartini J C
Vet Immunol Immunopathol. 1985 Jan;8(1-2):15-25. doi: 10.1016/0165-2427(85)90106-0.
Interleukin-2 (IL-2), a T cell derived lymphokine, acts in nonspecific hormone-like fashion to maintain proliferation of activated lymphocytes in vitro and is believed to play a key role in cell-mediated immune function in vivo. The parameters of induction and assay of factors with IL-2 activity were examined in a group of clinically normal sheep seronegative for antibodies to ovine progressive pneumonia virus (OPPV-). Supernatants from cultures of Concanavalin A (Con A) stimulated mononuclear leukocytes (ML) derived from peripheral blood and lymph nodes contained factors with the capacity to maintain continued proliferation in Con A stimulated lymphoblasts. This activity was localized by gel chromatography to fractions containing proteins of 17,000-20,000 daltons. In a group of sheep seropositive for antibodies to OPPV (OPPV+), decreased levels of IL-2 activity were found in ML culture supernatants derived from the posterior mediastinal lymph nodes of sheep with clinical and pathological evidence of OPP, when compared to OPPV+ sheep with no lesions and sheep with visceral caseous lymphadenitis. This decrease in IL-2 activity appeared not to be associated directly with levels of prostaglandin E2 in these supernatants. These findings may correlate with virus induced alterations in cell mediated immune function in lymphoproliferative lesions of OPP.
白细胞介素-2（IL-2）是一种由T细胞产生的淋巴因子，以非特异性激素样方式发挥作用，在体外维持活化淋巴细胞的增殖，并且被认为在体内细胞介导的免疫功能中起关键作用。在一组对绵羊进行性肺炎病毒（OPPV-）抗体血清学阴性的临床正常绵羊中，检测了具有IL-2活性的因子的诱导和测定参数。来自外周血和淋巴结的伴刀豆球蛋白A（Con A）刺激的单核白细胞（ML）培养物的上清液中含有能够维持Con A刺激的淋巴母细胞持续增殖的因子。这种活性通过凝胶色谱法定位到含有17,000-20,000道尔顿蛋白质的级分中。在一组对OPPV抗体血清学阳性（OPPV+）的绵羊中，与无病变的OPPV+绵羊和患有内脏干酪样淋巴结炎的绵羊相比，在具有OP临床和病理证据的绵羊后纵隔淋巴结来源的ML培养上清液中发现IL-2活性水平降低。IL-2活性的这种降低似乎与这些上清液中前列腺素E2的水平没有直接关联。这些发现可能与OP淋巴增殖性病变中病毒诱导的细胞介导免疫功能改变相关。
