Anandan Jayasree, Shanmugam Rajeshkumar
Nanobiomedicine Laboratory, Centre for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Apr 8;16(4):e57824. doi: 10.7759/cureus.57824. eCollection 2024 Apr.
Background Dental caries stands out as a significant global infectious disease, with oral diseases posing substantial health concerns primarily due to bacterial, fungal, and yeast infections. demonstrates antimicrobial, anticancer, antiparasitic, and hepatoprotective properties, with applications in various ailments. exhibits potent antimicrobial effects against bacterial and viral pathogens due to the bioactive compounds within the plant. This study aims to assess the antimicrobial efficacy of and formulation against oral pathogens and evaluate its other biomedical potential. Methodology The agar well diffusion method was employed to assess the antimicrobial activity of the formulation containing and against oral pathogens. The protein leakage assay was employed to assess the ability of the prepared formulation to cause protein release from oral pathogens. The other biomedical potentials of the prepared formulation including cytotoxic effects, antioxidant, and anti-inflammatory properties were investigated using in vitro assays. Results The prepared and formulation demonstrated significant antimicrobial activity against tested oral pathogens, with inhibition zones observed for (32 mm), (22 mm), and (12 mm). However, no inhibition was observed on at the highest concentration of 100 μL. Additionally, the formulation demonstrated significant antioxidant activity with percentages of 89.22%, 84.4%, and 86.93% in 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (HO), and ferric (Fe)-reducing antioxidant power assays, respectively, at the maximum concentration of 50 μL. Furthermore, the formulation exhibited potential anti-inflammatory activity, as evidenced by 79% inhibition in bovine serum albumin (BSA) denaturation assay and 77% inhibition in egg albumin (EA) denaturation assay at the highest concentration of 50 μL. Additionally, the formulation displayed low cytotoxic effects, even at the highest concentration of 80 μL. Conclusion and formulation demonstrated potential antimicrobial efficacy against oral pathogens and exhibited diverse therapeutic potentials. Thus, the developed formulation could be used as a potential alternative for pharmaceutical drugs against oral pathogens.
龋齿是一种严重的全球性传染病，口腔疾病主要由于细菌、真菌和酵母菌感染而引发重大健康问题。[某物质]具有抗菌、抗癌、抗寄生虫和保肝特性，可用于多种疾病。由于植物中的生物活性化合物，[某物质]对细菌和病毒病原体具有强大的抗菌作用。本研究旨在评估[某物质]与[另一物质]配方对口腔病原体的抗菌效果，并评估其其他生物医学潜力。
采用琼脂扩散法评估含[某物质]与[另一物质]的配方对口腔病原体的抗菌活性。采用蛋白质泄漏试验评估所制备配方导致口腔病原体释放蛋白质的能力。使用体外试验研究所制备配方的其他生物医学潜力，包括细胞毒性作用、抗氧化和抗炎特性。
所制备的[某物质]与[另一物质]配方对测试的口腔病原体显示出显著的抗菌活性，观察到[具体病原体1]的抑菌圈为32毫米，[具体病原体2]为22毫米，[具体病原体3]为12毫米。然而，在最高浓度100微升时，对[具体病原体4]未观察到抑制作用。此外，该配方在最大浓度50微升时，在2,2-二苯基-1-苦基肼（DPPH）、过氧化氢（HO）和铁（Fe）还原抗氧化能力试验中分别显示出89.22%、(84.4%)和86.93%的显著抗氧化活性。此外，该配方表现出潜在的抗炎活性，在最高浓度50微升时，牛血清白蛋白（BSA）变性试验中抑制率为79%，卵清蛋白（EA）变性试验中抑制率为77%。此外，即使在最高浓度80微升时，该配方也显示出低细胞毒性作用。
[某物质]与[另一物质]配方对口腔病原体显示出潜在的抗菌效果，并具有多种治疗潜力。因此，所开发的配方可作为对抗口腔病原体的药物的潜在替代品。
