Stefanowicz-Hajduk Justyna, Hering Anna, Kowalczyk Mariusz, Hałasa Rafał, Gucwa Magdalena, Ochocka J Renata
Department of Biology and Pharmaceutical Botany, Medical University of Gdańsk, 80-416 Gdansk, Poland.
Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Pulawy, Poland.
Plants (Basel). 2023 Jun 10;12(12):2268. doi: 10.3390/plants12122268.
species are succulents occurring in tropical regions. They have many biological and pharmacological properties. In this study, the cytotoxic and antimicrobial activities of water and dichloromethane fractions obtained from ethanol extracts of three species-, , and were estimated. The cytotoxic effect was assessed on human cancer cell lines-ovarian SKOV-3, cervical HeLa, breast MCF-7, and melanoma A375-by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antimicrobial activity was estimated on selected Gram-positive and Gram-negative bacteria strains and on . The phytochemical analysis of selected extracts was conducted by LC-QTOF-MS. The obtained results showed that the water fraction of was active both on the tested cancer cells (IC values were 28.28 ± 2.76 and 32.51 ± 0.69 µg/mL on HeLa and SKOV-3, respectively) and bacteria strains (MIC values were 16 and 32 µg/mL on and , respectively). The water fraction of also had a significant effect on and , with MIC values of 32 and 64 µg/mL, respectively. The water fraction of triggered a decrease in mitochondrial membrane potential (MMP) and induced cell cycle arrest in the G2/M phase in the SKOV-3 and HeLa cells. This fraction did not significantly increase cellular oxidative stress level. The DPPH and ABTS assays revealed that the water fraction of had a strong antioxidant effect (IC was 9.44 ± 0.06 and 3.17 ± 0.1 µg/mL, respectively). The phytochemical analysis of the extracts of and revealed the presence of at least 218 main components. The most frequently occurring were flavonol glycosides (31 metabolites), phenylpropanoids (13 metabolites), gallic acid derivatives (13 compounds), benzoic acid derived compounds (14 metabolites), and acyclic alcohol glycosides (16 compounds). In addition, proanthocyanidins were detected mainly in The study indicates that the water fraction of has significant biological potential and can be further investigated towards anticancer and antimicrobial application.
这些物种是生长在热带地区的多肉植物。它们具有许多生物学和药理学特性。在本研究中，对从三种物种（、和）的乙醇提取物中获得的水相和二氯甲烷相的细胞毒性和抗菌活性进行了评估。通过MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）试验评估对人癌细胞系（卵巢SKOV-3、宫颈HeLa、乳腺MCF-7和黑色素瘤A375）的细胞毒性作用。对选定的革兰氏阳性和革兰氏阴性细菌菌株以及进行抗菌活性评估。通过LC-QTOF-MS对选定提取物进行植物化学分析。所得结果表明，的水相对测试的癌细胞（对HeLa和SKOV-3的IC值分别为28.28±2.76和32.51±0.69µg/mL）和细菌菌株（对和的MIC值分别为16和32µg/mL）均有活性。的水相对和也有显著影响，MIC值分别为32和64µg/mL。的水相导致SKOV-3和HeLa细胞线粒体膜电位（MMP）降低，并诱导细胞周期停滞在G2/M期。该部分没有显著增加细胞氧化应激水平。DPPH和ABTS试验表明，的水相具有很强的抗氧化作用（IC分别为9.44±0.06和3.17±0.1µg/mL）。对和提取物的植物化学分析显示至少存在218种主要成分。最常见的是黄酮醇苷（31种代谢物）、苯丙烷类（13种代谢物）、没食子酸衍生物（13种化合物）、苯甲酸衍生化合物（14种代谢物）和无环醇苷（16种化合物）。此外，原花青素主要在中检测到。该研究表明，的水相具有显著的生物学潜力，可进一步研究其在抗癌和抗菌方面的应用。
