Coutelier J P, Van Snick J
Eur J Immunol. 1985 Mar;15(3):250-5. doi: 10.1002/eji.1830150308.
Lactic dehydrogenase elevating virus (LDV) was found to selectively stimulate IgG2a synthesis in infected mice. Within one week after infection, the production of IgG2a increased nearly 50-fold whereas that of IgM, IgA, IgG1 and IgG3 remained virtually unchanged. IgG2b synthesis was also enhanced but to a lesser extent. Several observations suggested that this stimulation of IgG2 production resulted from a polyclonal B cell activation: (a) the isoelectric focusing patterns of IgG2a before and after LDV infection were exactly the same, (b) the frequency of clones with anti-LDV activity in hybridoma collections derived from infected mice was extremely low (less than 4/1000) and (c) the proliferative response elicited by LDV in unsensitized animals was comparable with that induced by lipopolysaccharide. The effect of LDV on immunoglobulin synthesis was drastically reduced in nude mice but was not affected by the X-linked B lymphocyte defect of animals carrying the xid mutation.
乳酸脱氢酶升高病毒（LDV）被发现可选择性地刺激受感染小鼠的IgG2a合成。感染后一周内，IgG2a的产量增加了近50倍，而IgM、IgA、IgG1和IgG3的产量几乎保持不变。IgG2b的合成也有所增强，但程度较小。多项观察结果表明，这种对IgG2产生的刺激是由多克隆B细胞活化引起的：（a）LDV感染前后IgG2a的等电聚焦模式完全相同，（b）在源自受感染小鼠的杂交瘤集合中，具有抗LDV活性的克隆频率极低（低于4/1000），以及（c）LDV在未致敏动物中引发的增殖反应与脂多糖诱导的反应相当。LDV对免疫球蛋白合成的影响在裸鼠中大幅降低，但不受携带xid突变动物的X连锁B淋巴细胞缺陷的影响。
