Hara T, Umeda T, Niijima T, Okabe T
J Cancer Res Clin Oncol. 1985;109(2):103-6. doi: 10.1007/BF00391883.
The human promyelocytic leukemia cell line HL60, when cultured in medium containing dimethyl sulfoxide (DMSO) or granulocyte colony-stimulating factor (G-CSF), stopped dividing and differentiated into cells with granulocyte characteristics. We found that differentiated HL60 cells have no detectable cytolytic activity against cultured human bladder cell line (T24 cell), as measured by release of (3H) thymidine, or against K562 cells, as measured by release of chromium-51. Differentiated HL60 cells inhibited incorporation of (3H) thymidine into the DNA of adherent T24 cells. Decreased incorporation was not caused by detachment of the target T24 cells from the culture wells. The degree of cytostasis was dependent on the E/T ratio, with a 70%-80% inhibition usually reached at the E/T ratio of 200:1. A wide variety of target cells was also shown to be sensitive to differentiated HL60 cell-mediated cytostasis.
人早幼粒细胞白血病细胞系HL60，当在含有二甲基亚砜（DMSO）或粒细胞集落刺激因子（G-CSF）的培养基中培养时，停止分裂并分化为具有粒细胞特征的细胞。我们发现，通过（3H）胸腺嘧啶核苷释放量测定，分化后的HL60细胞对培养的人膀胱细胞系（T24细胞）没有可检测到的细胞溶解活性；通过51铬释放量测定，对K562细胞也没有细胞溶解活性。分化后的HL60细胞抑制（3H）胸腺嘧啶核苷掺入贴壁T24细胞的DNA中。掺入量的减少不是由靶T24细胞从培养孔中脱离引起的。细胞生长抑制程度取决于效应细胞与靶细胞比例（E/T比），在E/T比为200:1时通常达到70%-80%的抑制率。还显示多种靶细胞对分化后的HL60细胞介导的细胞生长抑制敏感。
