Raaberg L, Nexø E, Damsgaard Mikkelsen J, Seier Poulsen S
Department of Gynaecology and Obstetrics, Hvidovre Hospital, Denmark.
Histochemistry. 1988;89(4):351-6. doi: 10.1007/BF00500636.
The tissue localisation and time of first appearance of Epidermal Growth Factor (EGF) in the developing rat were investigated by means of immunohistochemistry, radioimmunoassay and radioreceptor assay. In this study we were able to show, that EGF appears prenatally in the lung and the kidney from gestational day 19. Postnatally EGF was found also in the gastrointestinal tract, first in Brunner's glands of the duodenum (at birth), next in the Paneth cells (day 7), and finally in the submandibular glands (day 25). The immunohistochemical and radioreceptor results are consistent, whereas the radioimmunoassay detects EGF later and in smaller quantities, than does the radioreceptor assay. These differences will be discussed.
通过免疫组织化学、放射免疫测定和放射受体测定法，对发育中的大鼠体内表皮生长因子（EGF）的组织定位和首次出现时间进行了研究。在本研究中，我们能够证明，从妊娠第19天起，EGF在肺和肾中产前出现。产后在胃肠道中也发现了EGF，首先在十二指肠的布伦纳腺（出生时），其次在潘氏细胞（第7天），最后在下颌下腺（第25天）。免疫组织化学和放射受体测定结果一致，而放射免疫测定法检测到EGF的时间比放射受体测定法晚，且数量较少。将对这些差异进行讨论。
