Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
State Key Laboratory of Petroleum Molecular and Process Engineering, SKLPMPE, Sinopec Research Institute of Petroleum Processing Co., Ltd., Beijing 100083, China.
Langmuir. 2024 May 21;40(20):10718-10725. doi: 10.1021/acs.langmuir.4c00727. Epub 2024 May 10.
For accurate in vivo detection, nonspecific adsorption of biomacromolecules such as proteins and cells is a severe issue. The adsorption leads to electrode passivation, significantly compromising both the sensitivity and precision of sensing. Meanwhile, common antibiofouling modifications, such as polymer coatings, still grapple with issues related to biocompatibility, electrode passivation, and miniaturization. Herein, we propose a composite antibiofouling coating strategy based on zwitterionic metal-organic frameworks (Z-MOFs) and a combination of acrylamide hydrogels. On a well-designed TiO/Z-MOF/hydrogel photoelectrode, we achieve highly sensitive and selective detection of dopamine in complex biological environments. The hydrogel's three-dimensional porous structure combined with unique microporous architecture of Z-MOF ensures effective sieving of interfering macromolecules while preserving efficient small molecules and electron transport. This innovative approach paves the way for constructing miniature, in vivo antibiofouling sensors for molecule monitoring in living organisms with complicated chemical environments.
为了实现准确的体内检测，生物大分子（如蛋白质和细胞）的非特异性吸附是一个严重的问题。这种吸附会导致电极钝化，极大地影响传感的灵敏度和精度。同时，常见的抗生物污染修饰方法，如聚合物涂层，仍然存在与生物相容性、电极钝化和小型化相关的问题。在这里，我们提出了一种基于两性离子金属-有机骨架（Z-MOFs）和丙烯酰胺水凝胶组合的复合抗生物污染涂层策略。在精心设计的 TiO/Z-MOF/水凝胶光电极上，我们实现了在复杂生物环境中对多巴胺的高灵敏度和选择性检测。水凝胶的三维多孔结构与 Z-MOF 的独特微孔结构相结合，确保了有效筛除干扰大分子，同时保留了高效小分子和电子传输。这种创新方法为构建用于在复杂化学环境中监测生物体内分子的微型、体内抗生物污染传感器铺平了道路。
