College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China.
College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China.
Food Chem. 2024 Sep 1;451:139295. doi: 10.1016/j.foodchem.2024.139295. Epub 2024 Apr 9.
Information regarding protein expression and phosphorylation modifications in the bovine milk fat globule membrane is scarce, particularly throughout various lactation periods. This study employed a complete proteome and phosphoproteome between bovine colostrum and mature milk. A total of 11 proteins were seen in both protein expression and phosphorylation levels. There were 400 proteins identified in only protein expression, and 104 phosphoproteins identified in only phosphorylation levels. A total of 232 significant protein characteristics were identified within the proteome and significant phosphorylation sites within 86 phosphoproteins of the phosphoproteome. Biological activities and pathways primarily exhibited associations with the immune system. Simultaneously, a comprehensive analysis of proteins and phosphorylation sites using a multi-omics approach. Hence, the data we have obtained has the potential to expand our understanding of how the bovine milk fat globule membrane might be utilized as a beneficial component in dairy products.
牛初乳和成熟乳之间的完整蛋白质组和磷酸化蛋白质组研究。在蛋白质表达和磷酸化水平上均观察到 11 种蛋白质。仅在蛋白质表达中鉴定出 400 种蛋白质，仅在磷酸化水平上鉴定出 104 种磷酸化蛋白质。在蛋白质组中鉴定出 232 个显著的蛋白质特征，在磷酸化蛋白质组中的 86 个磷酸化蛋白质中鉴定出显著的磷酸化位点。生物活性和途径主要与免疫系统相关。同时，使用多组学方法对蛋白质和磷酸化位点进行全面分析。因此，我们获得的数据有可能扩展我们对牛乳脂肪球膜如何可能作为乳制品中的有益成分的理解。
