Chen Ao, Yang Qianyu, Ye Wen, Xu Lingna, Wang Yuzhan, Sun Dongxiao, Han Bo
Department of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Beijing Jingwa Agricultural Innovation Center, Beijing 100193, China.
Animals (Basel). 2024 Apr 24;14(9):1276. doi: 10.3390/ani14091276.
Our preliminary research proposed the cytochrome P450 family 7 subfamily A member 1 () and hydroxyacyl-coenzyme A dehydrogenase trifunctional multienzyme complex beta subunit () genes as candidates for association with milk-production traits in dairy cattle because of their differential expression across different lactation stages in the liver tissues of Chinese Holstein cows and their potential roles in lipid metabolism. Hence, we identified single-nucleotide polymorphisms (SNPs) of the and genes and validated their genetic effects on milk-production traits in a Chinese Holstein population with the goal of providing valuable genetic markers for genomic selection (GS) in dairy cattle, This study identified five SNPs, 14:g.24676921A>G, 14:g.24676224G>A, 14:g.24675708G>T, 14:g.24665961C>T, and 14:g.24664026A>G, in the gene and three SNPs, 11:g.73256269T>C, 11:g.73256227A>C, and 11:g.73242290C>T, in . The single-SNP association analysis revealed significant associations ( value ≤ 0.0461) between the eight SNPs of and genes and 305-day milk, fat and protein yields. Additionally, using Haploview 4.2, we found that the five SNPs of formed two haplotype blocks and that the two SNPs of formed one haplotype block; notably, all three haplotype blocks were also significantly associated with milk, fat and protein yields ( value ≤ 0.0315). Further prediction of transcription factor binding sites (TFBSs) based on Jaspar software (version 2023) showed that the 14:g.24676921A>G, 14:g.24675708G>T, 11:g.73256269T>C, and 11:g.73256227A>C SNPs could alter the 5' terminal TFBS of the and genes. The 14:g.24665961C>T SNP caused changes in the structural stability of the mRNA for the gene. These alterations have the potential to influence gene expression and, consequently, the phenotype associated with milk-production traits. In summary, we have confirmed the genetic effects of and genes on milk-production traits in dairy cattle and identified potential functional mutations that we suggest could be used for GS of dairy cattle and in-depth mechanistic studies of animals.
我们的初步研究提出，细胞色素P450家族7亚家族A成员1（）和羟酰基辅酶A脱氢酶三功能多酶复合体β亚基（）基因可能与奶牛产奶性状相关，这是因为它们在中国荷斯坦奶牛肝脏组织的不同泌乳阶段存在差异表达，且在脂质代谢中具有潜在作用。因此，我们鉴定了和基因的单核苷酸多态性（SNP），并在中国荷斯坦牛群体中验证了它们对产奶性状的遗传效应，目的是为奶牛的基因组选择（GS）提供有价值的遗传标记。本研究在基因中鉴定出5个SNP，分别为14:g.24676921A>G、14:g.24676224G>A、14:g.24675708G>T、14:g.24665961C>T和14:g.24664026A>G；在中鉴定出3个SNP，分别为11:g.73256269T>C、11:g.73256227A>C和11:g.73242290C>T。单SNP关联分析显示，和基因的8个SNP与305天产奶量、乳脂产量和乳蛋白产量之间存在显著关联（值≤0.0461）。此外，使用Haploview 4.2软件，我们发现基因的5个SNP形成了两个单倍型块，基因的2个SNP形成了一个单倍型块；值得注意的是，所有三个单倍型块也与产奶量、乳脂产量和乳蛋白产量显著相关（值≤0.0315）。基于Jaspar软件（2023版）对转录因子结合位点（TFBS）的进一步预测表明，14:g.24676921A>G、14:g.24675708G>T、11:g.73256269T>C和11:g.73256227A>C这几个SNP可能会改变和基因的5'末端TFBS。14:g.24665961C>T这个SNP导致了基因mRNA结构稳定性的变化。这些改变有可能影响基因表达，进而影响与产奶性状相关的表型。总之，我们证实了和基因对奶牛产奶性状的遗传效应，并鉴定出了潜在的功能性突变，我们认为这些突变可用于奶牛的基因组选择以及动物的深入机制研究。
