Chen Huapu, Li Zhiyuan, Wang Yaorong, Huang Hai, Yang Xuewei, Li Shuangfei, Yang Wei, Li Guangli
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources, Hainan Tropical Ocean University, Ministry of Education, Sanya 572022, China.
Animals (Basel). 2021 Apr 7;11(4):1042. doi: 10.3390/ani11041042.
is a new and emerging aquaculture species in south China. However, due to the lack of understanding of reproductive regulation, the management of breeding and reproduction under captivity remains a barrier for the commercial aquaculture of . More genetic information is needed to identify genes critical for gonadal development. Here, the first gonadal transcriptomes of were analyzed and 151.89 million clean reads were generated. All reads were assembled into 57,077 unigenes, and 24,574 could be annotated. By comparing the gonad transcriptomes, 11,487 differentially expressed genes were obtained, of which 4599 were upregulated and 6888 were downregulated in the ovaries. Using enrichment analyses, many functional pathways were found to be associated with reproduction regulation. A set of sex-biased genes putatively involved in gonad development and gametogenesis were identified and their sexually dimorphic expression patterns were characterized. The detailed transcriptomic data provide a useful resource for further research on reproductive manipulation.
是中国南方一种新兴的水产养殖品种。然而，由于对生殖调控缺乏了解，圈养条件下的繁殖管理仍然是其商业水产养殖的一个障碍。需要更多的遗传信息来鉴定对性腺发育至关重要的基因。在此，分析了该品种的首个性腺转录组，共产生了1.5189亿条 clean reads。所有 reads 被组装成57077个单基因，其中24574个可以被注释。通过比较性腺转录组，获得了11487个差异表达基因，其中4599个在卵巢中上调，6888个下调。通过富集分析，发现许多功能途径与生殖调控有关。鉴定出一组可能参与性腺发育和配子发生的性别偏向基因，并对其性别二态性表达模式进行了表征。详细的转录组数据为进一步研究该品种的生殖操作提供了有用的资源。
