中药防风多糖的结构特征及免疫调节活性研究
Structure characterization and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix.
机构信息
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China; College of Bioengineering, Beijing Polytechnic, Beijing 100029, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
出版信息
Int J Biol Macromol. 2023 Apr 1;233:123502. doi: 10.1016/j.ijbiomac.2023.123502. Epub 2023 Feb 1.
A new polysaccharide, named SP800201 with Mw of 2.17 × 10 g/mol, was isolated from Saposhnikoviae Radix. The monosaccharide composition of SP800201 mainly contained Gal, GalA, Ara, and Rha. SP800201 has a core structure containing GalA as the backbone and side chains consisting of GalA, Gal, Ara and Rha. Cell and zebrafish experiments were used to explore the immunomodulatory activity of SP800201. Results of vitro RAW264.7 cell experiments showed that SP800201 could significantly improve the proliferation and phagocytosis of macrophages, and promote the release of NO, TNF-α, IL-1β, and IL-6. Results of vivo experiments in immunocompromised zebrafish showed that SP800201 could also significantly increase the density of immune cells, the number of macrophages, and reduce NO, TNF-α, IL-1β, and IL-6. The above results showed that the Saposhnikoviae Radix polysaccharide has certain immunomodulatory activity.
一种新的多糖，命名为 SP800201，Mw 为 2.17×10 g/mol，从防风根中分离得到。SP800201 的单糖组成主要含有半乳糖醛酸（GalA）、半乳糖（Gal）、阿拉伯糖（Ara）和鼠李糖（Rha）。SP800201 具有一个核心结构，包含半乳糖醛酸（GalA）作为主链，以及由半乳糖醛酸（GalA）、半乳糖（Gal）、阿拉伯糖（Ara）和鼠李糖（Rha）组成的侧链。通过细胞和斑马鱼实验来探究 SP800201 的免疫调节活性。体外 RAW264.7 细胞实验结果表明，SP800201 可显著提高巨噬细胞的增殖和吞噬作用，并促进 NO、TNF-α、IL-1β 和 IL-6 的释放。免疫功能低下斑马鱼体内实验结果表明，SP800201 还可以显著增加免疫细胞密度、巨噬细胞数量，并降低 NO、TNF-α、IL-1β 和 IL-6。上述结果表明，防风根多糖具有一定的免疫调节活性。
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