Pharmacy College of Xinjiang Medical University, Urumqi 830011, China.
Pharmacy College of Xinjiang Medical University, Urumqi 830011, China.
Int J Biol Macromol. 2017 Dec;105(Pt 1):452-462. doi: 10.1016/j.ijbiomac.2017.07.060. Epub 2017 Jul 12.
A water-soluble polysaccharide named ESBP2-1 was isolated from Erythronium sibiricum bulb through anion-exchange and size-exclusion chromatography. ESBP2-1 is a neutral polysaccharide fraction with an average molecular weight of 9.4×10Da and is composed of glucose, galactose and arabinose in a ratio of 24.3:1.1:1. Methylation analysis, partial hydrolysis and NMR studies revealed that the backbone of ESBP2-1 primarily consists of repeating →1)-α-d-Glcp-(4→ units where the disaccharide side chains of Arap-(1→6)-α-d-Galp-(1→ residue are attached to the O-6 position of Glc. An in vitro assay showed that ESBP2-1 significantly promoted the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells. In addition, ESBP2-1 stimulated the production of secretory molecules (nitric oxide, TNF-α and IL-1β) of RAW264.7 cells in a dose-dependent manner. These results suggest that ESBP2-1 is a potential immunostimulator.
从西伯利亚杓兰鳞茎中分离出水溶性多糖 ESBP2-1。ESBP2-1 是一种中性多糖，平均分子量为 9.4×10Da，由葡萄糖、半乳糖和阿拉伯糖以 24.3:1.1:1 的比例组成。甲基化分析、部分水解和 NMR 研究表明，ESBP2-1 的骨架主要由重复的→1)-α-d-Glcp-(4→单元组成，其中 Arap-(1→6)-α-d-Galp-(1→残基的二糖侧链连接到 Glc 的 O-6 位。体外试验表明，ESBP2-1 可显著促进 RAW 264.7 巨噬细胞的增殖和中性红吞噬作用。此外，ESBP2-1 以剂量依赖的方式刺激 RAW264.7 细胞分泌分子（一氧化氮、TNF-α 和 IL-1β）的产生。这些结果表明 ESBP2-1 是一种潜在的免疫刺激剂。
