Shockley R K, Jay W M, Fishman P H, Aziz M Z, Rissing J P
Acta Ophthalmol (Copenh). 1985 Feb;63(1):35-8. doi: 10.1111/j.1755-3768.1985.tb05212.x.
A reproducible animal model is necessary to examine the use of antimicrobial agents for prophylaxis and treatment of bacterial endophthalmitis. We determined the minimum inoculum size of S. aureus and P. aeruginosa that consistently produced endophthalmitis when injected into aphakic rabbit eyes immediately following surgery. Both anterior chamber and intravitreal injections were examined. For S. aureus, an intravitreal inoculum of 19.3 +/- 7.5 CFU and an anterior chamber inoculum of 50.5 +/- 4.0 CFU were required. For P. aeruginosa, an intravitreal inoculum of 5.5 +/- 2.6 CFU and an anterior chamber inoculum of 97.5 +/- 10.7 CFU consistently produced a fulminant infection. Lower inocula of both bacteria produced endophthalmitis in both locations, but the effect was inconsistent.
为了研究抗菌药物在预防和治疗细菌性眼内炎中的应用，需要建立一种可重复的动物模型。我们确定了金黄色葡萄球菌和铜绿假单胞菌的最小接种量，即在手术后立即将其注入无晶状体兔眼时能持续引发眼内炎的接种量。对前房注射和玻璃体内注射均进行了研究。对于金黄色葡萄球菌，玻璃体内接种量为19.3±7.5 CFU，前房接种量为50.5±4.0 CFU。对于铜绿假单胞菌，玻璃体内接种量为5.5±2.6 CFU，前房接种量为97.5±10.7 CFU时可持续产生暴发性感染。两种细菌较低的接种量在两个部位均引发了眼内炎，但效果并不一致。
