Department of Pharmacological and Biomolecular Sciences "Rodolfo Paoletti", University of Milan, 20133 Milan, Italy.
Consorzio Castanicoltori di Brinzio, Orino e Castello Cabiaglio, Società Cooperativa Agricola-Varese, 21100 Varese, Italy.
Int J Mol Sci. 2024 Apr 27;25(9):4764. doi: 10.3390/ijms25094764.
Acne vulgaris is a prevalent skin disorder affecting many young individuals, marked by keratinization, inflammation, seborrhea, and colonization by (). Ellagitannins, known for their antibacterial and anti-inflammatory properties, have not been widely studied for their anti-acne effects. Chestnut ( Mill., ), a rich ellagitannin source, including castalagin whose acne-related bioactivity was previously unexplored, was investigated in this study. The research assessed the effect of leaf extract and castalagin on human keratinocytes (HaCaT) infected with , finding that both inhibited IL-8 and IL-6 release at concentrations below 25 μg/mL. The action mechanism was linked to NF-κB inhibition, without AP-1 involvement. Furthermore, the extract displayed anti-biofilm properties and reduced CK-10 expression, indicating a potential role in mitigating inflammation, bacterial colonization, and keratosis. Castalagin's bioactivity mirrored the extract's effects, notably in IL-8 inhibition, NF-κB inhibition, and biofilm formation at low μM levels. Other polyphenols, such as flavonol glycosides identified via LC-MS, might also contribute to the extract's biological activities. This study is the first to explore ellagitannins' potential in treating acne, offering insights for developing chestnut-based anti-acne treatments pending future in vivo studies.
寻常痤疮是一种常见的皮肤疾病，影响许多年轻人，其特征为角质化、炎症、皮脂溢和 ()定植。鞣花单宁因其具有抗菌和抗炎特性而备受关注，但尚未广泛研究其抗痤疮作用。栗（Mill.）是鞣花单宁的丰富来源，包括此前未探索过其与痤疮相关的生物活性的苛伐鞣灵，本研究对其进行了调查。研究评估了栗叶提取物和苛伐鞣灵对感染的人角质形成细胞（HaCaT）的影响，发现两者均能在低于 25 μg/mL 的浓度下抑制 IL-8 和 IL-6 的释放。作用机制与 NF-κB 抑制有关，与 AP-1 无关。此外，提取物显示出抗生物膜特性，并降低 CK-10 表达，表明其在减轻炎症、细菌定植和角化方面具有潜在作用。苛伐鞣灵的生物活性与提取物的作用相似，特别是在低 μM 水平下抑制 IL-8、抑制 NF-κB 和生物膜形成。通过 LC-MS 鉴定的其他多酚类化合物，如类黄酮糖苷，也可能对提取物的生物活性有贡献。本研究首次探讨了鞣花单宁在治疗痤疮方面的潜力，为开发基于栗的抗痤疮治疗方法提供了依据，有待进一步的体内研究。
