Piazza Stefano, Martinelli Giulia, Vrhovsek Urska, Masuero Domenico, Fumagalli Marco, Magnavacca Andrea, Pozzoli Carola, Canilli Luisa, Terno Massimo, Angarano Marco, Dell'Agli Mario, Sangiovanni Enrico
Department of Pharmacological and Biomolecular Sciences, University of Milan, 20133 Milan, Italy.
Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), 38098 S. Michele all'Adige, Italy.
Antioxidants (Basel). 2022 Jun 5;11(6):1119. doi: 10.3390/antiox11061119.
() is recognized as one of the main triggers of the cutaneous inflammatory response in acne vulgaris, a chronic skin disorder with a multifactorial origin. Witch hazel ( L.) is a plant widely used for skin inflammatory conditions, with some preliminary anti-inflammatory evidence on the skin, but lacking data on acne conditions. This study aimed to evaluate the effect of a glycolic extract from bark (HVE) versus -induced inflammation in human keratinocytes (HaCaT). Phytochemical investigations of HVE identified hamamelitannin (HT) and proanthocyanidins as the most abundant compounds (respectively, 0.29% and 0.30% /). HVE inhibited -induced IL-6 release (IC: 136.90 μg/mL), by partially impairing NF-κB activation; however, no antibacterial or antibiofilm activities were found. In addition, HVE showed greater anti-inflammatory activity when TNF-α was used as a proinflammatory stimulus (IC of 38.93 μg/mL for IL-8 release), partially acting by antioxidant mechanisms, as shown for VEGF inhibition. The effects of HVE are primarily based on the proanthocyanidin content, as HT was found inactive on all the parameters tested. These results suggest further investigations of HVE in other inflammatory-based skin diseases.
（）被认为是寻常痤疮皮肤炎症反应的主要触发因素之一，寻常痤疮是一种起源多因素的慢性皮肤疾病。金缕梅（L.）是一种广泛用于皮肤炎症性疾病的植物，在皮肤上有一些初步的抗炎证据，但缺乏关于痤疮情况的数据。本研究旨在评估金缕梅树皮乙醇提取物（HVE）对人角质形成细胞（HaCaT）中脂多糖诱导的炎症的影响。对HVE的植物化学研究确定了金缕梅鞣质（HT）和原花青素是最丰富的化合物（分别为0.29%和0.30%/）。HVE通过部分损害NF-κB激活来抑制脂多糖诱导的IL-6释放（IC：136.90μg/mL）；然而，未发现抗菌或抗生物膜活性。此外，当TNF-α用作促炎刺激时，HVE表现出更大的抗炎活性（IL-8释放的IC为38.93μg/mL），部分通过抗氧化机制起作用，如对VEGF抑制所示。HVE的作用主要基于原花青素含量，因为发现HT对所有测试参数均无活性。这些结果表明需要进一步研究HVE在其他基于炎症的皮肤疾病中的作用。
