Pichyangkul S, Miller J E, Waldrop S, Khan A
Clin Immunol Immunopathol. 1985 Apr;35(1):22-34. doi: 10.1016/0090-1229(85)90074-1.
The ability of various subsets of human mononuclear cells to produce human lymphotoxin (LT) was examined. Peripheral blood mononuclear cells were separated into OKT4+, OKT8+, and Leu-11a+ subpopulations by flow cytometry. Both OKT4+ and OKT8+ cells produced LT upon phytohemagglutinin (PHA) stimulation, but the OKT4+ T cells were the major source of LT. In contrast, Leu-11a+ cells failed to produce LT. The LT production and the proliferative response to PHA, of OKT4+ and OKT8+ cells, were inhibited by prostaglandin E2 and histamine. The LT, derived from PHA-stimulated peripheral blood lymphocytes, was purified by a procedure involving Blue-agarose, Con A-Sepharose chromatography, preparative gradient polyacrylamide gel electrophoresis and preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The LT activity was recovered from SDS gel with major activity peak in the Mr 76,000 region and the minor activity peak in the Mr 24,000 region. The LT derived from 1788 lymphoblastoid cell line also showed heterogeneity on SDS gel. The activity was recovered from two peaks in the region of Mr 70,000 and 20,000.
研究了人类单核细胞不同亚群产生人淋巴毒素（LT）的能力。通过流式细胞术将外周血单核细胞分离为OKT4 +、OKT8 +和Leu - 11a +亚群。OKT4 +和OKT8 +细胞在植物血凝素（PHA）刺激下均产生LT，但OKT4 + T细胞是LT的主要来源。相比之下，Leu - 11a +细胞不能产生LT。OKT4 +和OKT8 +细胞的LT产生及对PHA的增殖反应受到前列腺素E2和组胺的抑制。通过涉及蓝琼脂糖、伴刀豆球蛋白A - 琼脂糖层析、制备性梯度聚丙烯酰胺凝胶电泳和制备性十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳的方法，纯化了来自PHA刺激的外周血淋巴细胞的LT。从SDS凝胶中回收LT活性，主要活性峰位于Mr 76,000区域，次要活性峰位于Mr 24,000区域。源自1788淋巴母细胞系的LT在SDS凝胶上也显示出异质性。活性从Mr 70,000和20,000区域的两个峰中回收。
