Suppr超能文献

家养草食动物 7 个物种 100 种组织的从头转录组组装数据库。

De novo transcriptome assembly database for 100 tissues from each of seven species of domestic herbivore.

机构信息

State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi, 832000, P. R. China.

College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, P. R. China.

出版信息

Sci Data. 2024 May 11;11(1):488. doi: 10.1038/s41597-024-03338-5.

Abstract

Domesticated herbivores are an important agricultural resource that play a critical role in global food security, particularly as they can adapt to varied environments, including marginal lands. An understanding of the molecular basis of their biology would contribute to better management and sustainable production. Thus, we conducted transcriptome sequencing of 100 to 105 tissues from two females of each of seven species of herbivore (cattle, sheep, goats, sika deer, horses, donkeys, and rabbits) including two breeds of sheep. The quality of raw and trimmed reads was assessed in terms of base quality, GC content, duplication sequence rate, overrepresented k-mers, and quality score distribution with FastQC. The high-quality filtered RNA-seq raw reads were deposited in a public database which provides approximately 54 billion high-quality paired-end sequencing reads in total, with an average mapping rate of ~93.92%. Transcriptome databases represent valuable resources that can be used to study patterns of gene expression, and pathways that are related to key biological processes, including important economic traits in herbivores.

摘要

家养草食动物是一种重要的农业资源,对全球粮食安全起着至关重要的作用,尤其是因为它们能够适应不同的环境,包括边缘土地。了解它们生物学的分子基础将有助于更好的管理和可持续生产。因此,我们对 7 种草食动物(牛、羊、山羊、梅花鹿、马、驴和兔子)的每一种的 2 只雌性个体的 100 到 105 个组织进行了转录组测序,其中包括 2 个绵羊品种。使用 FastQC 从碱基质量、GC 含量、重复序列率、过度代表的 k-mer 和质量分数分布等方面评估了原始和修剪后的读取质量。高质量过滤的 RNA-seq 原始读取被存入一个公共数据库,该数据库总共提供了约 540 亿个高质量的双端测序读取,平均映射率约为 93.92%。转录组数据库是一种有价值的资源,可以用来研究基因表达模式,以及与关键生物学过程相关的途径,包括草食动物的重要经济性状。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a587/11088706/8db1b11ee270/41597_2024_3338_Fig1_HTML.jpg

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验