School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Microb Cell Fact. 2024 May 12;23(1):135. doi: 10.1186/s12934-024-02413-1.
Biotin, serving as a coenzyme in carboxylation reactions, is a vital nutrient crucial for the natural growth, development, and overall well-being of both humans and animals. Consequently, biotin is widely utilized in various industries, including feed, food, and pharmaceuticals. Despite its potential advantages, the chemical synthesis of biotin for commercial production encounters environmental and safety challenges. The burgeoning field of synthetic biology now allows for the creation of microbial cell factories producing bio-based products, offering a cost-effective alternative to chemical synthesis for biotin production. This review outlines the pathway and regulatory mechanism involved in biotin biosynthesis. Then, the strategies to enhance biotin production through both traditional chemical mutagenesis and advanced metabolic engineering are discussed. Finally, the article explores the limitations and future prospects of microbial biotin production. This comprehensive review not only discusses strategies for biotin enhancement but also provides in-depth insights into systematic metabolic engineering approaches aimed at boosting biotin production.
生物素在羧化反应中作为辅酶，是人类和动物自然生长、发育和整体健康所必需的重要营养物质。因此，生物素广泛应用于饲料、食品和制药等多个行业。尽管具有潜在的优势，但用于商业生产的生物素的化学合成在环境和安全方面存在挑战。新兴的合成生物学领域现在允许创建生产基于生物的产品的微生物细胞工厂，为生物素生产提供了一种比化学合成更具成本效益的替代方案。本文概述了生物素生物合成涉及的途径和调控机制。然后，讨论了通过传统化学诱变和先进代谢工程来提高生物素产量的策略。最后，文章探讨了微生物生物素生产的局限性和未来前景。本文全面综述了生物素增强策略，同时深入探讨了旨在提高生物素产量的系统代谢工程方法。
