Sladká M, Kremen J, Kren V
Neoplasma. 1985;32(2):161-7.
Changes of karyotype in spontaneous acute lymphoblastic Lewis rat leukemia have been studied by conventional Giemsa staining method and by G- and C-banding techniques. Comparing with previously published normal findings in first passages on rats, an increasing number of breaks, gaps and fragments in the 5th and 14th passages has been proved. Chromosomal investigation performed after two-year transplantation of leukemia on syngenic animals revealed pseudodiploid karyotype of leukemic lymphoblasts with the persistence of cell line 42, XX, del 2, -7, -18, +2 mar, that remained unchanged up to the latest examination in April 1984. Remarkable stability of chromosomal changes such as del 2 and 7/18 translocation might indicate possible insertion sites of transforming (viral) agent and/or localization of putative oncogenes.
采用传统吉姆萨染色法以及G带和C带技术，对自发急性淋巴细胞性Lewis大鼠白血病的核型变化进行了研究。与先前发表的大鼠初代传代正常结果相比，已证实第5代和第14代出现的断裂、裂隙和片段数量增多。在同基因动物上进行白血病两年移植后进行的染色体研究显示，白血病淋巴母细胞的假二倍体核型，伴有42, XX, del 2, -7, -18, +2 mar细胞系持续存在，直至1984年4月的最新检查时仍未改变。诸如del 2和7/18易位等染色体变化的显著稳定性，可能表明转化（病毒）因子的可能插入位点和/或推定癌基因的定位。
