Tan Shaohua, Li Xiangchen, Lu Bing, Lin Yibin, Cai Yunxiang, He Jie, Chen Sisi, Gao Junli, Gao Junshun, Qiang Xinhua
Department of Clinical Laboratory, The First People's Hospital of Huzhou, Huzhou, Zhejiang, 313000, People's Republic of China.
Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Hangzhou, Zhejiang, 310020, People's Republic of China.
Infect Drug Resist. 2024 May 7;17:1781-1790. doi: 10.2147/IDR.S458625. eCollection 2024.
Carbapenem-resistant pose a significant threat to public health, causing gastroenteritis and invasive infections. We report the first emergence of a carbapenem-resistant serovar London strain, A132, carrying the gene in China. Whole-genome sequencing and bioinformatics analysis assigned A132 to be ST155, a multidrug-resistant clone frequently reported in China. The strain A132 exhibited resistance to multiple antibiotics, with 20 acquired antibiotic resistance genes (ARGs) identified, predominantly located on the IncFIB plasmid (pA132-1-NDM). Notably, the gene was located within an IS flanked-class 1 integron-IS complex, comprising two genetic cassettes. One cassette is the class 1 integron, which may facilitate the transmission of the entire complex, while the other is the -containing ISISflanked cassette, carrying multiple other ARGs. Genbank database search based on the -carrying cassette identified a similar genetic context found in transmissible IncFIA plasmids from (p91) and (p388) with a shared host range, suggesting the potential for cross-species transmission of . To our knowledge, this is the first reported case of serovar London ST155 harboring gene. Phylogenetic analysis indicated a close relationship between A132 and eight . London ST155 strains isolated from the same province. However, A132 differed by carrying the gene and four unique ARGs. Given the high transmissibility of the F-type plasmid harboring and 18 other ARGs, it is imperative to implement vigilant surveillance and adopt appropriate infection control measures to mitigate the threat to public health.
耐碳青霉烯类对公共卫生构成重大威胁，可导致肠胃炎和侵袭性感染。我们报告了在中国首次出现携带该基因的耐碳青霉烯类血清型伦敦菌株A132。全基因组测序和生物信息学分析将A132归类为ST155，这是一种在中国经常报道的多重耐药克隆。菌株A132对多种抗生素表现出耐药性，共鉴定出20个获得性抗生素耐药基因（ARGs），主要位于IncFIB质粒（pA132 - 1 - NDM）上。值得注意的是，该基因位于一个由IS侧翼的1类整合子 - IS复合物内，该复合物包含两个基因盒。一个基因盒是1类整合子，它可能促进整个复合物的传播，而另一个是含有的ISIS侧翼基因盒，携带多个其他ARGs。基于携带该基因盒的Genbank数据库搜索发现，在来自（p91）和（p388）的可传播IncFIA质粒中发现了相似的遗传背景，且具有共同的宿主范围，这表明该基因具有跨物种传播的潜力。据我们所知，这是首次报道血清型伦敦ST155携带该基因的病例。系统发育分析表明A132与从同一省份分离出的8个血清型伦敦ST155菌株关系密切。然而，A132因携带该基因和4个独特的ARGs而有所不同。鉴于携带该基因和其他18个ARGs的F型质粒具有高传播性，必须实施 vigilant surveillance并采取适当的感染控制措施，以减轻对公共卫生的威胁。
原文中“vigilant surveillance”未翻译，可能是原文有误，推测可能是“vigilant surveillance”（严密监测）。
