Jabbour Rabih E, Kang Joshua S, Sobhi Hany F
U.S. Army Edgewood Chemical Biological Center, Research & Technology Directorate, 5183 Blackhawk Rd, Aberdeen Proving Ground, Aberdeen, Maryland 21010, United States.
Center for Organic Synthesis, Department of Natural Sciences, Coppin State University, Baltimore, Maryland 21216, United States.
ACS Omega. 2024 Apr 24;9(18):20003-20011. doi: 10.1021/acsomega.3c10053. eCollection 2024 May 7.
Bacterial nanocellulose (BNC) biofilms, produced by various bacterial species, such as , represent a highly promising multifunctional material characterized by distinctive physiochemical properties. These biofilms have demonstrated remarkable versatility as nano biomaterials, finding extensive applications across medical, defense, electronics, optics, and food industries. In contrast to plant cellulose, BNC biofilms exhibit numerous advantages, including elevated purity and crystallinity, expansive surface area, robustness, and excellent biocompatibility, making them exceptional multifunctional materials. However, their production with consistent morphological properties and their transformation into practical forms present challenges. This difficulty often arises from the heterogeneity in cell density, which is influenced by the presence of -acyl-homoserine lactones (AHLs) serving as quorum sensing signaling molecules during the biosynthesis of BNC biofilms. In this study, we employed surface characterization methodologies including scanning electron microscopy, energy-dispersive spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy, and atomic force microscopy to characterize BNC biofilms derived from growth media supplemented with varying concentrations of distinct -acyl-homoserine lactone signaling molecules. The data obtained through these analytical techniques elucidated that the morphological properties of the BNC biofilms were influenced by the specific AHLs, signaling molecules, introduced into the growth media. These findings lay the groundwork for future exploration of leveraging synthetic biology and biomimetic methods for tailoring BNC with predetermined morphological properties.
由多种细菌物种（如 ）产生的细菌纳米纤维素（BNC）生物膜是一种极具潜力的多功能材料，具有独特的物理化学性质。这些生物膜作为纳米生物材料已展现出卓越的多功能性，在医疗、国防、电子、光学和食品工业等领域有着广泛应用。与植物纤维素相比，BNC生物膜具有诸多优势，包括更高的纯度和结晶度、更大的表面积、更强的坚固性以及出色的生物相容性，使其成为特殊的多功能材料。然而，以一致的形态特性生产BNC生物膜并将其转化为实际应用形式面临挑战。这种困难通常源于细胞密度的不均匀性，而细胞密度在BNC生物膜生物合成过程中受到作为群体感应信号分子的 -酰基高丝氨酸内酯（AHLs）的影响。在本研究中，我们采用了包括扫描电子显微镜、能量色散光谱、漫反射红外傅里叶变换光谱和原子力显微镜在内的表面表征方法，对源自添加了不同浓度不同 -酰基高丝氨酸内酯信号分子的生长培养基的BNC生物膜进行表征。通过这些分析技术获得的数据表明，BNC生物膜的形态特性受到引入生长培养基中的特定AHLs（信号分子）的影响。这些发现为未来利用合成生物学和仿生方法定制具有预定形态特性的BNC奠定了基础。
