Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
Department of Biomaterials, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai 600077, India.
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006383.
Two strictly aerobic and rod-shaped bacteria, labelled as DB1703 and DB2414S, were obtained from an automobile air conditioning system. Strain DB1703 was Gram-stain-negative, while strain DB2414S was Gram-stain-positive. Both strains were catalase-positive and oxidase-negative. Strains DB1703 and DB2414S were able to grow at 18-42 °C. Strain DB1703 grew within a NaCl range of 0-3 % and a pH range of 6.0-8.0; while strain DB2414S grew at 0-1 % and pH 6.5-8.5. The phylogenetic and 16S rRNA gene sequence analysis indicated that strains DB1703 and DB2414S belonged to the genera and , respectively. Strain DB1703 showed the closest phylogenetic similarity to YIM 100770 (94.8 %), whereas strain DB2414S was most closely related to ATCC BAA-1496 (97.7 %). The genome sizes of strains DB1703 and DB2414S were 4 652 148 and 4 282 418 bp, respectively, with DNA G+C contents of 68.8 and 70.5 mol%, respectively. Chemotaxonomic data showed Q-10 as the sole ubiquinone in DB1703 and ML-8 (H4) in DB2414S. The predominant cellular fatty acid in DB1703 was summed feature 8 (C 7 and/or C 6), whereas iso-C, C 8, and iso-C were dominant in DB2414S. Overall, the polyphasic taxonomic comparisons showed that strains DB1703 and DB2414S were distinct from their closest taxa and represent novel species within the genera and , respectively. Accordingly, we propose the names sp. nov., with the type strain DB1703 (=KCTC 72724=NBRC 114759), and sp. nov., with the type strain DB2414S (=KCTC 49355=NBRC 114620).
从汽车空调系统中分离得到两株严格需氧、呈杆状的细菌，分别命名为 DB1703 和 DB2414S。菌株 DB1703 革兰氏染色阴性，而 DB2414S 革兰氏染色阳性。两菌株均为过氧化氢酶阳性，氧化酶阴性。菌株 DB1703 可在 18-42°C 下生长，DB1703 可在 0-3%NaCl 和 pH6.0-8.0 范围内生长；而 DB2414S 可在 0-1%NaCl 和 pH6.5-8.5 范围内生长。系统发育和 16S rRNA 基因序列分析表明，菌株 DB1703 和 DB2414S 分别属于 和 属。菌株 DB1703 与 YIM 100770（94.8%）的亲缘关系最近，而 DB2414S 与 ATCC BAA-1496（97.7%）的亲缘关系最近。菌株 DB1703 和 DB2414S 的基因组大小分别为 4652148 和 4282418bp，GC 含量分别为 68.8%和 70.5%mol。化学分类学数据表明，菌株 DB1703 唯一的类异戊二烯醌为 Q-10，而 DB2414S 的为 ML-8（H4）。菌株 DB1703 的主要细胞脂肪酸为 summed feature 8（C7 和/或 C6），而 DB2414S 的主要细胞脂肪酸为 iso-C、C8 和 iso-C。总之，多相分类学比较表明，菌株 DB1703 和 DB2414S 与最接近的分类群明显不同，分别代表 和 属中的新种。因此，我们建议将菌株 DB1703 命名为 sp. nov.，其模式菌株为 DB1703（=KCTC 72724=NBRC 114759），将菌株 DB2414S 命名为 sp. nov.，其模式菌株为 DB2414S（=KCTC 49355=NBRC 114620）。
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