Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
J Pharm Biomed Anal. 2024 Jan 20;238:115856. doi: 10.1016/j.jpba.2023.115856. Epub 2023 Nov 8.
Farfarae Flos is a commonly used traditional herb for the treatment of respiratory disorders. In this study, ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry combined with the mass defect filter method was used for the qualitative analysis of Farfarae Flos metabolites in the lung tissues. Then a method for the simultaneous determination of 14 Farfarae Flos metabolites was developed and validated in terms of specificity, linearity, precision and accuracy, matrix effect and recovery. The method was applied to compare the lung tissue of Farfarae Flos treated mice, and 10 caffeoylquinic acid metabolites were higher in the mice with better antitussive effect. Further network pharmacology analysis and molecular docking results showed that these metabolites played an important role in the antitussive effect of Farfarae Flos. This study presented a novel strategy for deciphering the active compounds of herbal medicine by inter-individual variability of bioactivities.
款冬花是一种常用于治疗呼吸系统疾病的传统草药。本研究采用超高效液相色谱-飞行时间质谱联用结合质量亏损过滤法对款冬花在肺组织中的代谢产物进行定性分析。然后建立并验证了同时测定 14 种款冬花代谢产物的方法，考察了方法的专属性、线性、精密度与准确度、基质效应及提取回收率。采用该方法比较了款冬花处理的小鼠的肺组织，其中具有更好镇咳作用的小鼠体内 10 种咖啡酰奎宁酸类代谢物含量较高。进一步的网络药理学分析和分子对接结果表明，这些代谢产物在款冬花的镇咳作用中发挥了重要作用。该研究通过生物活性的个体间差异为解析草药的活性化合物提供了一种新策略。
