Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
J Pharm Biomed Anal. 2022 Aug 5;217:114836. doi: 10.1016/j.jpba.2022.114836. Epub 2022 May 17.
In this study, the inter-individual variability of antitussive effect of Farfarae Flos was observed, and then the Farfarae Flos treated mice were divided into the mice with good or poor antitussive effect. Then a UHPLC-Q TOF-MS method was developed and validated to quantify 13 fecal metabolites of Farfarae Flos, and the results showed concentrated differences between the two subgroups. The results of 16 S rRNA gene sequencing analysis showed that mice with good or poor antitussive effects were also different at the structure of gut microbiota in phylum and genus, as well as the related 6 pathways. In addition, the differential fecal metabolites of Farfarae Flos between the two subgroups were probably related with 5 bacterial that participating in the CQAs and flavonoids metabolism. This study explained the inter-individual variability of the antitussive effect of Farfarae Flos from the perspective of gut microbiota. However, the specific bacterial that participate in the metabolism of Farfarae Flos as well as the antitussive effects of Farfarae Flos need to be further validated.
在这项研究中，观察了款冬花镇咳作用的个体间变异性，然后将款冬花治疗的小鼠分为镇咳效果好或差的小鼠。然后建立并验证了一种 UHPLC-Q TOF-MS 方法来定量分析 13 种款冬花粪便代谢物，结果表明这两个亚组之间存在明显差异。16S rRNA 基因测序分析结果表明，镇咳效果好或差的小鼠在门和属水平的肠道微生物群结构以及相关的 6 条途径上也存在差异。此外，两个亚组之间款冬花的差异粪便代谢物可能与参与 CQAs 和类黄酮代谢的 5 种细菌有关。本研究从肠道微生物群的角度解释了款冬花镇咳作用的个体间变异性。然而，具体参与款冬花代谢以及款冬花镇咳作用的细菌还需要进一步验证。
