School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China.
School of Material Science and Engineering, Yancheng Institute of Technology, Yancheng, 224051, Jiangsu, China.
Mikrochim Acta. 2024 May 15;191(6):331. doi: 10.1007/s00604-024-06402-4.
A broad host range phage-based nanozyme (Fe-MOF@SalmpYZU47) was prepared for colorimetric detection of multiple Salmonella enterica strains. The isolation of a broad host range phage (SalmpYZU47) capable of infecting multiple S. enterica strains was achieved. Then, it was directly immobilized onto the Fe-MOF to prepare Fe-MOF@SalmpYZU47, exhibiting peroxidase-like activity. The peroxidase-like activity can be specifically inhibited by multiple S. enterica strains, benefiting from the broad host range capture ability of Fe-MOF@SalmpYZU47. Based on it, a colorimetric detection approach was developed for S. enterica in the range from 1.0 × 10 to 1.0 × 10 CFU mL, achieving a low limit of detection (LOD) of 11 CFU mL. The Fe-MOF@SalmpYZU47 was utilized for detecting S. enterica in authentic food samples, achieving recoveries ranging from 91.88 to 105.34%. Hence, our proposed broad host range phage-based nanozyme exhibits significant potential for application in the colorimetric detection of pathogenic bacteria.
一种基于广谱噬菌体的纳米酶（Fe-MOF@SalmpYZU47）被制备用于比色法检测多种沙门氏菌。实现了能够感染多种沙门氏菌的广谱噬菌体（SalmpYZU47）的分离。然后，它被直接固定在 Fe-MOF 上，制备出具有过氧化物酶样活性的 Fe-MOF@SalmpYZU47。过氧化物酶样活性可被多种沙门氏菌菌株特异性抑制，这得益于 Fe-MOF@SalmpYZU47 的广谱宿主捕获能力。基于此，开发了一种用于检测 1.0×10 到 1.0×10 CFU mL 范围内沙门氏菌的比色检测方法，检测限（LOD）低至 11 CFU mL。该 Fe-MOF@SalmpYZU47 被用于检测真实食品样品中的沙门氏菌，回收率在 91.88%至 105.34%之间。因此，我们提出的基于广谱噬菌体的纳米酶在用于致病性细菌的比色检测方面具有重要的应用潜力。
