State Key Laboratory of Agricultural Microbiology, National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen 518000, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China; Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China.
Food Chem. 2024 Jun 15;443:138539. doi: 10.1016/j.foodchem.2024.138539. Epub 2024 Jan 26.
Quinoxalines are a class of veterinary drugs with antibacterial and growth-promoting functions. They are often widely used to treat and prevent animal diseases and are illegally used as animal growth promoters to increase economic benefits. Quinoxalines could be easily metabolized in animals to various residue markers and remain in animal-derived foods, which would pose a serious threat to human health. Consequently, it is necessary to detect the residues of quinoxalines and their metabolites. This article reviewed and evaluated immunoassays for quinoxalines and their metabolites in animal-derived foods, mainly including enzyme-linked immunosorbent assays, fluorescence immunosorbent assays, immunochromatography, and surface plasmon resonance biosensors. In addition, we deeply explored the design of haptens for quinoxalines and their metabolites and analyzed the effect of haptens on antibody performance. This paper aims to provide guidance and references for their accurate and sensitive detection, thereby ensuring food safety and human public health.
喹喔啉类是一类具有抗菌和促生长功能的兽药。它们通常被广泛用于治疗和预防动物疾病，并被非法用作动物生长促进剂以提高经济效益。喹喔啉类在动物体内很容易代谢为各种残留标志物，并残留在动物源性食品中，这对人类健康构成严重威胁。因此，有必要检测喹喔啉类及其代谢物的残留。本文综述和评价了动物源性食品中喹喔啉类及其代谢物的免疫分析方法，主要包括酶联免疫吸附测定法、荧光免疫吸附测定法、免疫层析法和表面等离子体共振生物传感器。此外，还深入探讨了喹喔啉类及其代谢物半抗原的设计，并分析了半抗原对抗体性能的影响。本文旨在为其准确、灵敏的检测提供指导和参考，从而确保食品安全和人类公共健康。
