Department of Plant Biotechnology, Medicinal Plants Research Centre, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.
Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Fundam Clin Pharmacol. 2024 Oct;38(5):842-861. doi: 10.1111/fcp.13009. Epub 2024 May 15.
Diabetes mellitus poses a global health challenge, driving the need for innovative therapeutic solutions. Experimental methods play a crucial role in evaluating the efficacy of potential antidiabetic drugs, both in vitro and in vivo. Yet concerns about reproducibility persist, necessitating comprehensive reviews.
This review aims to outline experimental approaches for inducing diabetes and evaluating antidiabetic activity, synthesizing data from authoritative sources and academic literature.
We conducted a systematic search of prominent databases, including PubMed, ScienceDirect, and Scopus, to identify relevant articles spanning from 1943 to the present. A total of 132 articles were selected for inclusion in this review, focusing on in vitro and in vivo experimental validations of antidiabetic treatments.
Our review highlights the diverse array of experimental methods employed for inducing diabetes mellitus and evaluating antidiabetic interventions. From cell culture assays to animal models, researchers have employed various techniques to study the effectiveness of novel therapeutic agents.
This review provides a comprehensive guide to experimental approaches for assessing antidiabetic activity. By synthesizing data from a range of sources, we offer valuable insights into the current methodologies used in diabetes research. Standardizing protocols and enhancing reproducibility are critical for advancing effective antidiabetic treatments.
糖尿病是一个全球性的健康挑战，需要创新的治疗方法。实验方法在评估潜在抗糖尿病药物的疗效方面起着至关重要的作用，无论是在体外还是体内。然而，人们对可重复性的担忧依然存在，因此需要进行全面的综述。
本综述旨在概述诱导糖尿病和评估抗糖尿病活性的实验方法，综合权威来源和学术文献的数据。
我们对包括 PubMed、ScienceDirect 和 Scopus 在内的主要数据库进行了系统搜索，以确定从 1943 年至今的相关文章。共有 132 篇文章被纳入本综述，重点关注抗糖尿病治疗的体外和体内实验验证。
我们的综述强调了用于诱导糖尿病和评估抗糖尿病干预的多种实验方法。从细胞培养测定到动物模型，研究人员已经采用了各种技术来研究新型治疗剂的有效性。
本综述提供了评估抗糖尿病活性的实验方法的综合指南。通过综合来自各种来源的数据，我们深入了解了当前糖尿病研究中使用的方法。标准化方案和提高可重复性对于推进有效的抗糖尿病治疗至关重要。
