Nainu Firzan, Bahar Muhammad A, Habibie Habibie, Najib Ahmad, Zubair Muhammad S, Arba Muhammad, Asbah Asbah, Mudjahid Mukarram, Latada Nadila P, Filmaharani Filmaharani, Putri Annisa A
Department of Pharmacy, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia.
Magister Program in Pharmacy, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar, Indonesia.
Narra J. 2025 Apr;5(1):e1712. doi: 10.52225/narra.v5i1.1712. Epub 2025 Jan 20.
The escalating prevalence of diabetes represents a critical challenge to global health and quality of life. Indonesia, particularly the Sulawesi region, is home to a diverse array of endemic plants with potential as sources of novel antidiabetic compounds. However, traditional preclinical models for evaluating these candidates are limited by high costs and lengthy timelines. The aim of this study was to explore the antidiabetic potential of and extracts using as a novel, cost- effective and efficient in vivo model. Hyperglycemia was induced in larvae through a high-sugar diet, and the plant extracts were incorporated into the larval diets at concentrations ranging from 0.3125% to 2.5%. Phenotypic parameters, including body size, body weight, crawling activity, and hemolymph glucose levels, were evaluated, and the expression of metabolism-related genes (, and ) was analyzed using RT-qPCR. This study found that and extracts improved crawling activity and body size in hyperglycemic larvae. Notably, extract significantly reduced hemolymph glucose levels ( < 0.01), increased body weight ( < 0.01), and upregulated the expression of metabolic genes such as ( < 0.001), ( < 0.001), and ( < 0.0001). In contrast, extract showed less pronounced effects, highlighting the efficacy of extract in alleviating hyperglycemia and restoring metabolic homeostasis. The study highlights that extract demonstrated promising antidiabetic properties in the model, underscoring the utility of this model for early-stage antidiabetic drug screening and supporting further preclinical investigation into the therapeutic potential of for managing hyperglycemia.
糖尿病患病率的不断上升对全球健康和生活质量构成了严峻挑战。印度尼西亚，尤其是苏拉威西地区，拥有各种各样的本土植物，这些植物有潜力成为新型抗糖尿病化合物的来源。然而，用于评估这些候选植物的传统临床前模型受到高成本和长周期的限制。本研究的目的是使用一种新型、经济高效的体内模型来探索[植物名称1]和[植物名称2]提取物的抗糖尿病潜力。通过高糖饮食诱导斑马鱼幼体出现高血糖，然后将植物提取物以0.3125%至2.5%的浓度添加到幼体饮食中。评估了包括体长、体重、爬行活动和血淋巴葡萄糖水平在内的表型参数，并使用RT-qPCR分析了代谢相关基因（[基因名称1]、[基因名称2]和[基因名称3]）的表达。本研究发现，[植物名称1]和[植物名称2]提取物改善了高血糖斑马鱼幼体的爬行活动和体长。值得注意的是，[植物名称1]提取物显著降低了血淋巴葡萄糖水平（P<0.01），增加了体重（P<0.01），并上调了如[基因名称1]（P<0.001）、[基因名称2]（P<0.001）和[基因名称3]（P<0.0001）等代谢基因的表达。相比之下，[植物名称2]提取物的效果不太明显，突出了[植物名称1]提取物在缓解高血糖和恢复代谢稳态方面的功效。该研究强调，[植物名称1]提取物在斑马鱼模型中表现出有前景的抗糖尿病特性，强调了该模型在早期抗糖尿病药物筛选中的实用性，并支持对[植物名称1]治疗高血糖的治疗潜力进行进一步的临床前研究。
