Allansmith M R, Baird R S, Bloch K J
Exp Eye Res. 1985 Jun;40(6):797-804. doi: 10.1016/0014-4835(85)90124-1.
To examine the role in ocular tissue injury of anaphylaxis alone (as distinct from other mechanisms possibly present in a model involving immunization), a model of anaphylaxis was produced by injection of rabbit anti-rat IgE antibody into ocular adnexal tissues of rats. Adnexal swelling, seen within 15 min, disappeared by 6 hr. Vascular permeability and weight of adnexal tissues were increased at 0.5 hr and had returned to near normal levels by 6 hr. Histologic study revealed extensive degranulation of mast cells at 0.5 hr after injection and a return to normal by 24 hr. Neutrophils reached a level of 7400/mm3 at 6 hr and returned to normal by 24 hr. Macrophages accumulated by 24 hr in all tissues, including control eyes injected with normal serum. The similarity of results from this 'pure' model of anaphylaxis to results from a model produced by injecting antigen into ocular adnexal tissues of immunized rats suggests that anaphylactic mechanisms are responsible for the changes seen in the antigen-injected model.
为了研究单纯过敏反应（与免疫模型中可能存在的其他机制不同）在眼组织损伤中的作用，通过将兔抗大鼠IgE抗体注射到大鼠眼附属器组织中建立了过敏反应模型。附属器肿胀在15分钟内出现，6小时后消失。附属器组织的血管通透性和重量在0.5小时时增加，6小时时已恢复到接近正常水平。组织学研究显示，注射后0.5小时肥大细胞广泛脱颗粒，24小时时恢复正常。中性粒细胞在6小时时达到7400/mm³的水平，24小时时恢复正常。到24小时时，巨噬细胞在所有组织中积聚，包括注射正常血清的对照眼。这个“纯”过敏反应模型的结果与将抗原注射到免疫大鼠眼附属器组织所产生模型的结果相似，这表明过敏机制是抗原注射模型中所见变化的原因。
