Merkl Padryk, Sotiriou Georgios A
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet SE-17177 Stockholm Sweden
Nanoscale Adv. 2024 Apr 15;6(10):2586-2593. doi: 10.1039/d4na00021h. eCollection 2024 May 14.
High concentrations of ammonia in the human body can occur due to a wide variety of underlying causes such as liver cirrhosis and the symptoms of high ammonia concentrations are diffuse and hard to diagnose. The measurement of blood ammonia levels is an important diagnostic tool but is challenging to perform at the patient's bedside. Here, we present a plasmonic Ag nanoparticle-based ammonia sensor which provides a colorimetric optical readout and does not require specialised equipment. This is achieved using plasmonic Ag/SiO nanoparticles with the sensing mechanism that in the presence of OCl they rapidly degrade reducing their plasmonic extinction and losing their characteristic colour. However, if ammonia is also present in the system, it neutralises the OCl and thus the silver nanoparticles retain their plasmonic colour as can be measured by the naked eye or using a spectrometer. This sensing was further developed to enable measurements with animal serum as well as a implementing a facile "dip-stick" style paper-based sensor.
人体内高浓度的氨可能由多种潜在原因引起，如肝硬化，而高氨浓度的症状较为弥散，难以诊断。血氨水平的测量是一项重要的诊断工具，但在患者床边进行测量具有挑战性。在此，我们展示了一种基于等离子体银纳米颗粒的氨传感器，它提供比色光学读数，且不需要专门设备。这是通过等离子体Ag/SiO纳米颗粒实现的，其传感机制是在存在OCl的情况下它们会迅速降解，降低其等离子体消光并失去其特征颜色。然而，如果系统中也存在氨，它会中和OCl，因此银纳米颗粒会保留其等离子体颜色，这可以通过肉眼或使用光谱仪测量。这种传感技术得到了进一步发展，能够使用动物血清进行测量，并实现了一种简便的“试纸”式纸质传感器。
