Department of Chemistry, Capital Normal University, Beijing 100048, China.
ACS Sens. 2024 May 24;9(5):2317-2324. doi: 10.1021/acssensors.4c00633. Epub 2024 May 16.
Cu accelerates the viral-like propagation of α-synuclein fibrils and plays a key role in the pathogenesis of Parkinson's disease (PD). Therefore, the accurate detection of Cu is essential for the diagnosis of PD and other neurological diseases. The Cu detection process is impeded by substances that have similar electrochemical properties. In this study, graphdiyne (GDY), a new kind of carbon allotrope with strong electron-donating ability, was utilized for the highly selective detection of Cu by taking advantage of its outstanding adsorption capacity for Cu. Density functional theory (DFT) calculations show that Cu atoms are adsorbed in the cavity of GDY, and the absorption energy between Cu and C atoms is higher than that of graphene (GR), indicating that the cavity of GDY is favorable for the adsorption of Cu atoms and electrochemical sensing. The GDY-based electrochemical sensor can effectively avoid the interference of amino acids, metal ions and neurotransmitters and has a high sensitivity of 9.77 μA·μM·cm, with a minimum detectable concentration of 200 nM. During the investigating pathogenesis and therapeutic process of PD with α-synuclein as the diagnostic standard, the concentration of Cu in cells before and after L-DOPA and GSH treatments were examined, and it was found that Cu exhibits high potential as a biomarker for PD. This study not only harnesses the favorable adsorption of the GDY and Cu to improve the specificity of ion detection but also provide clues for deeper understanding of the role of Cu in neurobiology and neurological diseases.
铜加速α-突触核蛋白原纤维的病毒样传播，并在帕金森病（PD）的发病机制中发挥关键作用。因此，准确检测铜对于 PD 和其他神经疾病的诊断至关重要。铜的检测过程受到电化学性质相似的物质的阻碍。在这项研究中，利用具有强供电子能力的新型碳同素异形体——石墨炔（GDY），因其对铜具有出色的吸附能力，可用于高度选择性地检测铜。密度泛函理论（DFT）计算表明，铜原子被吸附在 GDY 的腔体内，并且 Cu 与 C 原子之间的吸收能高于石墨烯（GR），表明 GDY 的腔体能有利于 Cu 原子的吸附和电化学传感。基于 GDY 的电化学传感器可以有效避免氨基酸、金属离子和神经递质的干扰，具有 9.77 μA·μM·cm 的高灵敏度，最小检测浓度为 200 nM。在以α-突触核蛋白为诊断标准的 PD 发病机制和治疗过程研究中，检查了 L-DOPA 和 GSH 处理前后细胞中 Cu 的浓度，结果表明 Cu 作为 PD 的生物标志物具有很大的潜力。本研究不仅利用 GDY 和 Cu 的有利吸附来提高离子检测的特异性，还为深入了解 Cu 在神经生物学和神经疾病中的作用提供了线索。
