Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99 Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea.
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006389.
A bacterial strain designated MMS21-TAE1-1, capable of degrading paraoxon, was isolated from red pepper soil (36° 25' 26.0″ N, 126° 25' 47.0″ E) and subjected to polyphasic taxonomic characterisation. MMS21-TAE1-1 was an aerobic, non-motile and Gram-stain-positive bacterium. MMS21-TAE1-1 showed growth at 10-37 °C (optimum, 30 °C), at pH 4-10 (optimum, pH 7) and in the presence of 0-6 % NaCl (optimum, 0 %). On the basis of the results of 16S rRNA gene sequence analysis, MMS21-TAE1-1 could be assigned to the genus and shared the highest sequence similarities with NBRC 12136 (99.72 %), then with G2-1 (99.65 %) and DSM 20138 (99.17 %). However, the results of genome-based comparison using orthologous average nucleotide identity (orthoANI) and digital DNA-DNA hybridisation indicated that MMS21-TAE1-1 could be readily distinguished from all species of the genus with validly published names. The predominant menaquinone of MMS21-TAE1-1 was MK-9(H). The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylinositol, and unidentified glycolipids were also present. The major fatty acids were anteiso-C, anteiso-C, iso-C and iso-C. The chemotaxonomic properties of MMS21-TAE1-1 were generally consistent with those of members of the genus . The genome of MMS21-TAE1-1 contained genes related to degradation of aromatic compounds. It is evident from the results of this study that strain MMS21-TAE1-1 merits recognition as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MMS21-TAE1-1 (=KCTC 49652 = LMG 32368).
一株能够降解对氧磷的细菌菌株 MMS21-TAE1-1 从红辣椒土壤（北纬 36°25'26.0″，东经 126°25'47.0″）中分离出来，并进行了多相分类特征研究。MMS21-TAE1-1 是一种需氧、非运动性、革兰氏阳性细菌。MMS21-TAE1-1 在 10-37°C（最佳温度为 30°C）、pH 值 4-10（最佳 pH 值为 7）和 0-6%NaCl（最佳 0%）的条件下生长。基于 16S rRNA 基因序列分析的结果，MMS21-TAE1-1 可被分配到属中，与 NBRC 12136（99.72%）的序列相似度最高，然后与 G2-1（99.65%）和 DSM 20138（99.17%）的相似度最高。然而，使用直系同源平均核苷酸同一性（orthoANI）和数字 DNA-DNA 杂交进行基于基因组的比较的结果表明，MMS21-TAE1-1 可以很容易地与所有具有有效发表名称的属内种区分开来。MMS21-TAE1-1 的主要menaquinone 是 MK-9(H)。诊断性极性脂是双磷脂酰甘油和磷脂酰肌醇，还存在未鉴定的糖脂。主要脂肪酸是 anteiso-C、anteiso-C、iso-C 和 iso-C。MMS21-TAE1-1 的化学生物分类特性通常与属内成员一致。MMS21-TAE1-1 的基因组包含与芳香族化合物降解相关的基因。从这项研究的结果可以明显看出，菌株 MMS21-TAE1-1 值得被认为是代表属的一个新种，因此提出了新种的名称。模式菌株是 MMS21-TAE1-1（=KCTC 49652=LMG 32368）。
