College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China.
Laboratory of Food and Environmental Microbiology, Earth and Life Institute, UCLouvain, Louvain-la-Neuve, B-1348, Belgium.
Virology. 2024 Aug;596:110101. doi: 10.1016/j.virol.2024.110101. Epub 2024 May 7.
This study characterizes a newly isolated Demerecviridae phage, named vB_SalS_PSa2, belonging to the phage T5 group. The main variations between vB_SalS_PSa2 and T5 concern structural proteins related to morphology and host recognition. vB_SalS_PSa2 is infective to 19 out of the 25 tested Salmonella enterica (including the rare "Sendai" and "Equine" serotypes) and Escherichia coli isolates, most of them being multidrug resistant. vB_SalS_PSa2 displayed good thermal stability (4-60 °C) and broad pH stability (4.0-12.0). It also exhibited antibacterial activity against S. enterica sv. Paratyphi A Enb50 at 4 °C in milk during the whole tested period (5 d), and for 3-6 h at both 25 and 37 °C. Furthermore, vB_SalS_PSa2 was able to inhibit biofilm formation and to show degradation activity on mature biofilms of E. coli K12 and S. enterica sv. Paratyphi Enb50 in both LB and milk. Altogether, these results indicate that phage vB_SalS_PSa2 is a valuable candidate for controlling foodborne S. enterica and E. coli pathogens.
本研究描述了一种新分离的 De-merecviridae 噬菌体，命名为 vB_SalS_PSa2，属于 T5 噬菌体群。vB_SalS_PSa2 与 T5 之间的主要差异在于与形态和宿主识别相关的结构蛋白。vB_SalS_PSa2 可感染 25 株测试的沙门氏菌（包括罕见的“仙台”和“马”血清型）和大肠杆菌分离株中的 19 株，其中大多数为多药耐药菌。vB_SalS_PSa2 表现出良好的热稳定性（4-60°C）和宽 pH 稳定性（4.0-12.0）。它还表现出对牛奶中 4°C 下的 S. enterica sv. Paratyphi A Enb50 的抗菌活性，在整个测试期（5d）内，以及在 25°C 和 37°C 下 3-6h 内。此外，vB_SalS_PSa2 能够抑制大肠杆菌 K12 和 S. enterica sv. Paratyphi Enb50 的成熟生物膜的形成，并在 LB 和牛奶中显示出降解活性。总之，这些结果表明噬菌体 vB_SalS_PSa2 是控制食源性病原体沙门氏菌和大肠杆菌的有价值的候选物。
