Department of Food Science and Nutrition, University of Minnesota-Twin Cities, Saint Paul, MN, 55108, USA.
Division of Environmental Health Science, School of Public Health, University of Minnesota-Twin Cities, Minneapolis, MN, 55455, USA.
Arch Virol. 2024 Nov 27;169(12):255. doi: 10.1007/s00705-024-06190-5.
Five lytic bacteriophages specific for Salmonella enterica and Escherichia coli were isolated from wastewater in Minnesota. These phages, designated vB_Sal_EH1, vB_Sal_EH2, vB_Sal_EH3, vB_Sal_EH4, and vB_Sal_EH7, were characterized, and their genomes were sequenced. Phylogenetic analysis showed that they grouped within the genus Epseptimavirus, with genome sizes ranging from 108,554 to 115,218 bp. All five phages exhibited lytic activity against both S. enterica and Shiga-toxin-producing E. coli O157:H7. Transposon mutagenesis of the host genome identified the outer membrane protein BtuB as essential for phage infection, suggesting that it is a putative receptor. Genome sequence comparisons revealed genetic loci that are variable among the isolated phages and potentially influence their host specificity and virulence.
从明尼苏达州的废水中分离到了 5 种特异性针对沙门氏菌和大肠杆菌的裂解噬菌体。这些噬菌体被命名为 vB_Sal_EH1、vB_Sal_EH2、vB_Sal_EH3、vB_Sal_EH4 和 vB_Sal_EH7，对它们进行了特征描述，并对其基因组进行了测序。系统发育分析表明，它们属于 Epseptimavirus 属，基因组大小从 108554 到 115218bp 不等。这 5 种噬菌体都对沙门氏菌和产志贺毒素大肠杆菌 O157:H7 具有裂解活性。对宿主基因组的转座子诱变鉴定出外膜蛋白 BtuB 对噬菌体感染是必需的，这表明它是一个假定的受体。基因组序列比较揭示了分离噬菌体之间存在可变的遗传基因座，这些基因座可能影响它们的宿主特异性和毒力。
