Shahedi Abbas, Talebi Ali Reza, Mirjalili Aghdas, Pourentezari Majid
Department of Biology and Anatomical Sciences, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Reproductive Biology, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Clin Exp Reprod Med. 2021 Mar;48(1):27-33. doi: 10.5653/cerm.2020.03853. Epub 2021 Feb 18.
The chief outcome of testicular torsion in clinical and experimental contexts is testicular ischemia. Curcumin, a compound with anti-inflammatory and antioxidant properties, has fascinated researchers and clinicians for its promise in the treatment of fertility diseases.
Thirty-five fully grown male mice were randomly classified into five groups: control, sham, testicular torsion, treatment group 1 (testicular torsion+short-term curcumin), and treatment group 2 (testicular torsion+long-term curcumin). Thirty-five days later, spermatozoa from the right cauda epididymis were analyzed with regard to count and motility. Toluidine blue (TB), aniline blue (AB), and chromomycin A3 (CMA3) staining assays were used to evaluate the sperm chromatin integrity. In addition, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) test was used to assess apoptosis.
Treatment group 1 exhibited a remarkably elevated sperm count compared to the testicular torsion group. Additionally, notably lower sperm motility was found in the testicular torsion group compared to the control, treatment 1, and treatment 2 groups. Staining (CMA3, AB, and TB) and the TUNEL test indicated significantly greater testicular torsion in the torsion group compared to the control group (p<0.05). The data also revealed notably lower results of all sperm chromatin assays and lower apoptosis in both treatment groups relative to the testicular torsion group (p<0.05). Significantly elevated (p<0.05) AB and TB results were noted in treatment group 1 compared to treatment group 2.
Curcumin can compensate for the harmful effects of testicular ischemia and improve sperm chromatin quality in mice.
在临床和实验环境中，睾丸扭转的主要后果是睾丸缺血。姜黄素是一种具有抗炎和抗氧化特性的化合物，因其在治疗生育疾病方面的前景而吸引了研究人员和临床医生。
将35只成年雄性小鼠随机分为五组：对照组、假手术组、睾丸扭转组、治疗组1（睾丸扭转+短期姜黄素）和治疗组2（睾丸扭转+长期姜黄素）。35天后，分析右侧附睾尾部精子的数量和活力。使用甲苯胺蓝（TB）、苯胺蓝（AB）和嗜铬霉素A3（CMA3）染色试验评估精子染色质完整性。此外，使用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记（TUNEL）试验评估细胞凋亡。
与睾丸扭转组相比，治疗组1的精子数量显著增加。此外，与对照组、治疗组1和治疗组2相比，睾丸扭转组的精子活力明显较低。染色（CMA3、AB和TB）和TUNEL试验表明，与对照组相比，扭转组的睾丸扭转明显更严重（p<0.05）。数据还显示，与睾丸扭转组相比，两个治疗组的所有精子染色质检测结果均显著降低，细胞凋亡也更低（p<0.05）。与治疗组2相比，治疗组1的AB和TB结果显著升高（p<0.05）。
姜黄素可以弥补睾丸缺血的有害影响，并改善小鼠的精子染色质质量。
