Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Fiocruz, Rio de Janeiro, Brazil.
Methods Mol Biol. 2024;2775:359-365. doi: 10.1007/978-1-0716-3722-7_23.
Extracellular vesicles (EVs) are produced by all domains of life. In fungal pathogens, they participate in virulence mechanisms and/or induce protective immunity, depending on the pathogenic species. EVs produced by pathogenic members of the Cryptococcus genus mediate virulence, antifungal resistance, as well as humoral and cell-mediated immunity. The isolation of cryptococcal EVs has been laborious and time-consuming for years. In this chapter, we detail a fast protocol for the isolation and analysis of EVs produced by members of the Cryptococcus genus.
细胞外囊泡（EVs）由所有生命领域产生。在真菌病原体中，它们参与毒力机制和/或诱导保护性免疫，具体取决于致病物种。致病性隐球菌属成员产生的 EVs 介导毒力、抗真菌耐药性以及体液和细胞介导的免疫。多年来，隐球菌 EVs 的分离一直很费力且耗时。在本章中，我们详细介绍了一种快速分离和分析隐球菌属成员产生的 EVs 的方案。
