从伯氏疟原虫中分离出的一种裂解因子的脂质组成与活性
Lipid composition and activity of a lytic factor isolated from Plasmodium berghei.
作者信息
Boyer J M, D'Antonio L E, Schiavone W A
出版信息
Infect Immun. 1979 Sep;25(3):805-9. doi: 10.1128/iai.25.3.805-809.1979.
A fraction was obtained from Plasmodium berghei which induced hemolysis of the erythrocytes of mice and hamsters. This fraction, called lytic factor (LF), was found to be composed of a large amount of lipid material. An examination of the lipids showed the major lipids to be monoglycerides, diglycerides, triglycerides, fatty acids, long-chain alcohol, sterol, sterol ester, sterol glycoside, and two cerebrosides. The most abundant component found in the LF was sterol ester, followed in order by cerebrosides, sterol, and sterol glycoside. Lytic activity was found to be lost when samples were boiled for 5 min. An examination of the lipid composition of LF before and after boiling showed changes which may be useful in studies on the mechanism of activity of this factor. The fatty acid composition of the total lipid fraction of LF was examined by gas-liquid chromatography. The major fractions were 18:1 and 16:0 in unheated LF and 16:0 in the heated LF.
从伯氏疟原虫中获得了一种能引起小鼠和仓鼠红细胞溶血的组分。该组分称为溶血因子（LF），发现它由大量脂质物质组成。对脂质的检测表明，主要脂质为甘油单酯、甘油二酯、甘油三酯、脂肪酸、长链醇、固醇、固醇酯、固醇糖苷和两种脑苷脂。LF中含量最丰富的成分是固醇酯，其次依次是脑苷脂、固醇和固醇糖苷。当样品煮沸5分钟时，溶血活性丧失。对煮沸前后LF的脂质组成进行检测，发现了一些变化，这些变化可能有助于研究该因子的活性机制。通过气液色谱法检测了LF总脂质组分的脂肪酸组成。未加热的LF中主要组分是18:1和16:0，加热后的LF中主要组分是16:0。
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