Department of Horticulture, College of Agriculture, University of Al-Azhar (Assiut Branch), Assiut, 71524, Egypt.
Botany Department, Faculty of Science, Aswan University, Aswan, 81528, Egypt.
BMC Plant Biol. 2024 May 18;24(1):420. doi: 10.1186/s12870-024-04961-5.
Cold atmospheric plasma (CAP) is a physical technology with notable effects on living organisms. In the present study, tomato seeds (Solanum lycopersicum var. Bassimo Mill.) were exposed to CAP for various time intervals, ranging from 1 to 5 min, in both continuous and intermittent periods, and were compared with a control group that received no CAP treatment. Seedlings grown from treated seeds exhibited improvements in levels of growth traits, photosynthetic pigments, and metabolite contents when compared to the control group. Seedlings from seeds treated with S04 displayed significant increases in shoot and root lengths, by 32.45% and 20.60% respectively, compared to the control group. Moreover, seedlings from seeds treated with S01 showed a 101.90% increase in total protein, whereas those treated with S02 experienced a 119.52% increase in carbohydrate content. These findings highlight the substantial improvements in growth characteristics, photosynthetic pigments, and metabolite levels in seedlings from treated seeds relative to controls. Total antioxidant capacity was boosted by CAP exposure. The activities of enzymes including superoxide dismutase, catalase, and peroxidases were stimulated by S02 and exceeded control treatment by (177.48%, 137.41%, and 103.32%), respectively. Additionally, exposure to S04 increased the levels of non-enzymatic antioxidants like flavonoids, phenolics, saponins, and tannins over the control group (38.08%, 30.10%, 117.19%, and 94.44%), respectively. Our results indicate that CAP-seed priming is an innovative and cost-effective approach to enhance the growth, bioactive components, and yield of tomato seedlings.
冷等离子体（CAP）是一种对生物体具有显著影响的物理技术。在本研究中，番茄种子（Solanum lycopersicum var. Bassimo Mill.）暴露于 CAP 下不同时间间隔，从 1 分钟到 5 分钟，分别为连续和间歇期，并与未接受 CAP 处理的对照组进行比较。与对照组相比，从经过处理的种子中生长的幼苗在生长特性、光合色素和代谢物含量方面均有所提高。与对照组相比，用 S04 处理的种子的苗高和根长分别显著增加了 32.45%和 20.60%。此外，用 S01 处理的种子的总蛋白增加了 101.90%，而用 S02 处理的种子的碳水化合物含量增加了 119.52%。这些发现强调了处理种子的幼苗在生长特性、光合色素和代谢物水平方面相对于对照的显著提高。暴露于 CAP 提高了总抗氧化能力。S02 刺激了超氧化物歧化酶、过氧化氢酶和过氧化物酶等酶的活性，分别比对照处理高 177.48%、137.41%和 103.32%。此外，暴露于 S04 增加了非酶抗氧化剂如类黄酮、酚类、皂苷和单宁的水平，分别比对照组高 38.08%、30.10%、117.19%和 94.44%。我们的结果表明，CAP 种子引发是一种创新且具有成本效益的方法，可以提高番茄幼苗的生长、生物活性成分和产量。
