Department of Endocrinology 2, Central Hospital Affiliated to Shenyang Medical College, Tiexi District, Shenyang, Liaoning Province, China.
Department of Internal Medicine 1, Tacheng People's Hospital of Xinjiang, Tacheng, Xinjiang, China.
Biomol Biomed. 2024 Sep 6;24(5):1170-1177. doi: 10.17305/bb.2024.10237.
Hashimoto's thyroiditis (HT) is a prevalent autoimmune disease. We investigated the relationship of peripheral blood long noncoding RNA-plasmacytoma variant translocation 1 (lncRNA-PVT1) and microRNA (miR)-146a levels with Th17/Treg-related cytokines in HT patients and their clinical significance. Correlations of PVT1 and miR-146a with Th17/Treg-related cytokines were analyzed, and its clinical value in diagnosing HT was assessed. Results showed reduced lncRNA-PVT1 and interleukin (IL)-10 levels and increased miR-146a and IL-17 levels in HT patients. lncRNA-PVT1 negatively interrelated with miR-146a, IL-17, IL-23 and IL-6, and positively interrelated with IL-10; miR-146a positively correlated with IL-17, IL-23 and IL-6, but negatively correlated with IL-10 in HT patients. The area under the curve (AUC) of lncRNA-PVT1 and miR-146a levels for diagnosing HT were 0.822 and 0.844, respectively (sensitivity 88.73% and 86.62%, specificity 67.02% and 69.15%, cut-off values 0.76 and 2.73), with their combined detections yielding a higher AUC. Patients with poorly expressed lncRNA-PVT1 and highly expressed miR-146a had elevated HT incidence. lncRNA-PVT1 and miR-146a levels were also found to be an independent influencing factor for HT occurrence. Our findings suggest that HT patients have low peripheral blood lncRNA-PVT1 expression and high miR-146a expression. lncRNA-PVT1 and miR-146a level changes were correlated with Th17/Treg cytokine imbalance and could be a potential diagnostic tool and independent influencing factor for HT.
桥本甲状腺炎（HT）是一种常见的自身免疫性疾病。我们研究了外周血长链非编码 RNA-浆细胞瘤变异易位 1（lncRNA-PVT1）和 microRNA（miR）-146a 水平与 HT 患者 Th17/Treg 相关细胞因子的关系及其临床意义。分析了 PVT1 和 miR-146a 与 Th17/Treg 相关细胞因子的相关性，并评估了其在诊断 HT 中的临床价值。结果显示，HT 患者的 lncRNA-PVT1 和白细胞介素（IL）-10 水平降低，miR-146a 和 IL-17 水平升高。lncRNA-PVT1 与 miR-146a、IL-17、IL-23 和 IL-6 呈负相关，与 IL-10 呈正相关；miR-146a 与 HT 患者的 IL-17、IL-23 和 IL-6 呈正相关，与 IL-10 呈负相关。lncRNA-PVT1 和 miR-146a 水平诊断 HT 的曲线下面积（AUC）分别为 0.822 和 0.844（灵敏度为 88.73%和 86.62%，特异性为 67.02%和 69.15%，截断值为 0.76 和 2.73），联合检测的 AUC 更高。lncRNA-PVT1 表达水平低和 miR-146a 表达水平高的患者 HT 发生率升高。lncRNA-PVT1 和 miR-146a 水平也是 HT 发生的独立影响因素。我们的研究结果表明，HT 患者外周血 lncRNA-PVT1 表达降低，miR-146a 表达升高。lncRNA-PVT1 和 miR-146a 水平的变化与 Th17/Treg 细胞因子失衡相关，可能是 HT 的潜在诊断工具和独立影响因素。
