小鼠中对麻风分枝杆菌感染抗性的过继性细胞转移
Adoptive cell transfer of resistance to Mycobacterium leprae infections in mice.
作者信息
Lowe C, Brett S J, Rees R J
出版信息
Clin Exp Immunol. 1985 Aug;61(2):336-42.
Cells were transferred from mice intradermally vaccinated with killed Mycobacterium leprae to sublethally irradiated recipients. Unseparated cells from lymph nodes or spleens of M. leprae vaccinated mice were found to cause significant inhibition of the growth of a subsequent M. leprae challenge in mouse footpads for up to 26 weeks after vaccination. Vaccination with live BCG and cells transferred from BCG-vaccinated mice caused no significant inhibition of M. leprae growth in mouse footpads. Cell separation into fractions containing predominantly B and T lymphocytes showed that the inhibition of growth was due to M. leprae-sensitized T lymphocytes. M. leprae vaccinated mice were also skin tested with soluble M. leprae antigen and showed maximum delayed hypersensitivity responses 4 weeks after vaccination.
将用灭活麻风分枝杆菌皮内接种小鼠的细胞转移至接受亚致死剂量照射的受体小鼠。发现来自接种麻风分枝杆菌小鼠的未分离的淋巴结或脾脏细胞，在接种后长达26周内，对随后小鼠足垫中麻风分枝杆菌攻击的生长具有显著抑制作用。用活卡介苗接种以及转移来自接种卡介苗小鼠的细胞，对小鼠足垫中麻风分枝杆菌的生长没有显著抑制作用。将细胞分离成主要含B淋巴细胞和T淋巴细胞的组分，结果表明生长抑制是由于麻风分枝杆菌致敏的T淋巴细胞所致。对接种麻风分枝杆菌的小鼠还用可溶性麻风分枝杆菌抗原进行皮肤试验，结果显示接种后4周出现最大延迟超敏反应。
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