[Histochemical and electrophoretic determination of a selected element of the rat myocardium metabolism as exemplified by lactate dehydrogenase (LDH/E.C.1.1.1.27/) following administration of the preparation Biseptol 480 (co-trimoxazole)].

Histochemical and electrophoretic examinations of the effect of Biseptol 480 on lactate dehydrogenase in the rat heart muscle were carried out on white male rats of Wistar strain of about 300 g in weight. The rats were divided into 2 groups: experimental group of 10 rats and a control one of 5 rats. Experimental rats were given, by stomach tube, 80 g of Biseptol 480, suspended in 1 cm3 of distilled water for 7 consecutive d. Control rats were given 1 cm3 of distilled water for 7 consecutive d too. Samples were taken from the experimental and control rats to examine lactate dehydrogenase and to make isoenzyme separation. The lack of differences between the experimental rat samples and those control ones permitted to conclude that Biseptol 480 administered to rats, did not bring about significant histoenzymatic and electrophoretic changes suggesting a disbalance in the examined stage of metabolism in the rat heart muscle.