[Histochemical and electrophoretic studies of lactate dehydrogenase (LDH/E.C. 1.1.1.27/) activity in the rat liver following the administration of Biseptol 480 (co-trimoxazole)].

The present experiments are stimulated by some reports suggesting disturbances in the liver metabolism after application of Biseptol 480. 10 a ago, Biseptol has been widely used in the treatment of some inflammatory states of the respiratory and urinary systems, the alimentary canal, the genitals, and of some bacterial skin infection. The examinations were carried out on 15 white male rats of Wistar strain which were treated by 80 mg of the sulphonamide Biseptol 480 for 7 consecutive days, by stomach tube. Histochemical activity of lactate dehydrogenase (LDH) and isoenzymatic composition of LDH were estimated in the liver's experimental sections. The results show a decrease of LDH activity in the liver tissue and the absence of LDH-5 and LDH-1 fractions in the experimental electrophoregrams. They confirm some authors' supposition of some disturbances in the liver metabolism following application of Biseptol 480.